皱纹盘鲍肽聚糖识别蛋白在免疫防御中的作用

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室

	皱纹盘鲍肽聚糖识别蛋白在免疫防御中的作用
	陈钰莹 1,2,3; 韩怡静 1; 刘相全 2; 何金霞 2; 杨顶珑 1
刊名	渔业科学进展
	2022
卷号	43 期号:4 页码:234-242
关键词	皱纹盘鲍肽聚糖识别蛋白免疫防御
ISSN号	2095-9869
其他题名	A Peptidoglycan Recognition Protein (PGRP) from Haliotis discus hannai: Possible Roles in Antibacterial Properties
文献子类	期刊论文
英文摘要	In this study, peptide PGRP (designated HdPGRP) was identified and characterized from the abalone Haliotis discus hannai. Multiple alignments and phylogenetic analyses strongly suggested that HdPGRP is a new member of the PGRP superfamily and belongs to the short PGRP family, similar to peptides from other marine mollusks. The full length of HdPGRP is 1467 bp, encoding a polypeptide of 354 amino acids (aa) with a signal peptide (1~18 aa), an SH3b domain (93~160 aa), a typical PGRP domain (179~322 aa), and an Ami_2 domain (191~322 aa). In addition, four conserved Zn~(2+)-binding sites (H~(209), Y~(255), H~(318), and C~(330)) and five conserved amide-catalysis sites (H~(209), Y~(255), H~(318), T328, and C~(330)) were found in the HdPGRP sequence. In abalone, hdpgrp exhibited different tissue expression patterns, and was strongly expressed in the hepatopancreas, moderately expressed in hemocytes, mantle, and gills, and slightly expressed in muscle. Vibrio anguillarum is one of the main pathogens of H. discus hannai; after V. anguillarum infection, expression of hdpgrp in hemocytes showed a trend of first increasing and then decreasing, reaching a maximum at 24 h. Subsequently, expression of HdPGRP decreased, and there was no significant difference compared with the control group at 72 h, demonstrating that expression of HdPGRP had returned to normal levels. SDS-PAGE results showed that recombinant HdPGRP (rHdPGRP) has a molecular mass of 30 kDa, which is in line with the value predicted for HdPGRP. PGRPs usually have amidase activity, degrading peptidoglycan by hydrolyzing the amide bond that links peptide units to muramic acid residues of glycan strands. rHdPGRP exhibited Zn~(2+)-dependent amidase activity and catalyzed the degradation of insoluble peptidoglycan. In addition, rHdPGRP exhibited significant antibacterial activity against the gram-positive bacterium Micrococcus luteus in the logarithmic phase in the presence of Zn~(2+), indicating that the antibacterial activity of HdPGRP might be dependent on amidase activity. In summary, HdPGRP plays an important role in PGRP-mediated antibacterial mechanisms, especially for eliminating invading bacteria.
语种	中文
CSCD记录号	CSCD:7264311
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/34259]
专题	海岸带生物学与生物资源利用重点实验室
作者单位	1.中国科学院烟台海岸带研究所,山东烟台264003 2.山东省资源与环境研究院山东省海洋生态修复重点实验室,山东烟台264006; 3.上海海洋大学水产与生命学院,上海201306;
推荐引用方式 GB/T 7714	陈钰莹,韩怡静,刘相全,等. 皱纹盘鲍肽聚糖识别蛋白在免疫防御中的作用[J]. 渔业科学进展,2022,43(4):234-242.
APA	陈钰莹,韩怡静,刘相全,何金霞,&杨顶珑.(2022).皱纹盘鲍肽聚糖识别蛋白在免疫防御中的作用.渔业科学进展,43(4),234-242.
MLA	陈钰莹,et al."皱纹盘鲍肽聚糖识别蛋白在免疫防御中的作用".渔业科学进展 43.4(2022):234-242.