K252a inhibition of sperm viability for efficient crossbreeding of hermaphroditic bivalves

doi:10.1016/j.aquaculture.2023.739930

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室

	K252a inhibition of sperm viability for efficient crossbreeding of hermaphroditic bivalves
	Liu, Tian 3,6,7; Wei, Huilan 3; Zhang, Lijing 3; Liu, Liangjie 3; Li, Yajuan 3; Shu, Ya 3; Yang, Yaxin 3; Zhao, Baojun 3; Xing, Qiang 3; Zeng, Qifan 3,5
刊名	AQUACULTURE
	2023-12-15
卷号	577 页码:9
关键词	K252a Sperm motility Crossbreeding Simultaneous hermaphrodite Argopecten irradians Argopecten purpuratus
ISSN号	0044-8486
DOI	10.1016/j.aquaculture.2023.739930
通讯作者	Zhang, Lingling(lingling80@ouc.edu.cn)
英文摘要	Bivalve mollusks are economically important shellfish, representing 56.3% of aquaculture production. Cross-breeding is a popular approach for the genetic improvement of aquaculture species, including bivalves. However, it is difficult for simultaneous hermaphrodites due to the difficulty in collecting unfertilized oocytes. Male infertility or subfertility could be a solution. Considering that protein phosphorylation is critical in spermato-genesis, we investigated the effects of the protein kinase inhibitor K252a on germ cell development, gamete quality and embryonic/larval development of the simultaneously hermaphroditic scallop Argopecten irradians and evaluated its feasibility in crossbreeding. The results showed that 20% K252a injection at the gonad growth stage led to significant decreases in sperm number and progressive motility rate. Abnormal axoneme arrange-ment in flagella may have caused reduced sperm motility. Normal oocytes fertilized with K252a-treated sperm showed significant decreases in fertilization rate (38.24%), cleavage rate (83.42%) and D-stage survival rate (15.93%), and K252a-treated oocytes did not show obvious defects in early development. Interspecific hybrid-ization using K252a-treated A. irradians fertilized with Argopecten purpuratus sperm resulted in a 3.19-fold in-crease in the hybrid ratio (67%) over that of the control group (16%). Our study indicated that K252a influenced sperm development without affecting oocytes in A. irradians, indicating that K252a treatment may facilitate the mass production of crossbreeds and hybrids for simultaneous hermaphrodites. The approach reported here will greatly assist in genetic improvement programs for mollusk hermaphrodites.
WOS关键词	PROTEIN-KINASE-C ; MALE-INFERTILITY ; BAY SCALLOP ; MICE DEFICIENT ; GROWTH ; MOTILITY ; DIFFERENTIATION ; SURVIVAL ; FLAGELLA ; LARVAE
WOS研究方向	Fisheries ; Marine & Freshwater Biology
语种	英语
WOS记录号	WOS:001051039500001
资助机构	National Key Research and Devel-opment Program of China ; Na-tional Natural Science Foundation of China ; Key R amp; D Project of Shandong Province
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/33456]
专题	海岸带生物学与生物资源利用重点实验室
通讯作者	Zhang, Lingling
作者单位	1.Qingdao Agr Univ, Coll Marine Sci & Engn, Qingdao, Peoples R China 2.Southern Marine Sci & Engn Guangdong Lab Guangzhou, Guangzhou, Peoples R China 3.Ocean Univ China, MOE Key Lab Marine Genet & Breeding, Qingdao, Peoples R China 4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Ctr Ocean Mega Sci, Yantai, Peoples R China 5.Ocean Univ China, Sanya Oceanog Inst, Lab Trop Marine Germplasm Resources & Breeding Eng, Sanya, Peoples R China 6.Laoshan Lab, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China 7.Laoshan Lab, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Liu, Tian,Wei, Huilan,Zhang, Lijing,et al. K252a inhibition of sperm viability for efficient crossbreeding of hermaphroditic bivalves[J]. AQUACULTURE,2023,577:9.
APA	Liu, Tian.,Wei, Huilan.,Zhang, Lijing.,Liu, Liangjie.,Li, Yajuan.,...&Zhang, Lingling.(2023).K252a inhibition of sperm viability for efficient crossbreeding of hermaphroditic bivalves.AQUACULTURE,577,9.
MLA	Liu, Tian,et al."K252a inhibition of sperm viability for efficient crossbreeding of hermaphroditic bivalves".AQUACULTURE 577(2023):9.