基于形态学和宏条形码技术的南海西沙群岛浮游动物多样性的比较分析

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	基于形态学和宏条形码技术的南海西沙群岛浮游动物多样性的比较分析
	王方晗 1,3; 王雷 1; 孙婷婷 1,3; 董志军 1,2
刊名	应用海洋学学报
	2022
卷号	41 期号:2 页码:317-327
关键词	海洋生物学珊瑚礁浮游动物多样性宏条形码西沙群岛
ISSN号	2095-4972
其他题名	Comparative morphology and metabarcoding analysis of zooplankton diversity in Xisha Islands in the South China Sea
文献子类	期刊论文
英文摘要	With the largest sea area in the South China Sea, Xisha Islands are rich in marine resources. To understand the composition and distribution characteristics of zooplankton in this area, a comprehensive survey of 14 stations in Xisha Islands was conducted in May 2019. Morphological methods and metabarcoding methods based on 18S V9 sequencing was used to examine the composition of zooplankton samples. We found that the main zooplankton species in Xisha Islands were copepods, Malacostraca and Sagittoidea, of them the relative abundance was both high by morphological and molecular identification. The average zooplankton density in the 14 stations was 707.53 378.34 ind/m~3 while it varied with zooplankton abundance, species composition and dominant species among the stations. Compared with morphological identification (86 species from 11 phylum, 17 classes, 18 orders), many more species were obtained by 18S V9 identification (233 OTUs from 22 phylum, 46 classes, 85 orders) and the relative abundance of representative categories and diversity index showed significant correlation with morphological identification in most stations, indicating that the metabarcoding technique and morphological identification method have good comparability in the evaluation of species diversity, and metabarcoding technique exhibited high application potential in efficient assessment of marine biodiversity. However, due to molecular methodology for zooplankton species identification is still in its infant stage, the relevant database is still not complete, and a variety of methods are still needed for the accuracy diversity assessment of zooplankton.
语种	中文
CSCD记录号	CSCD:7193143
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/34253]
专题	中国科学院牟平海岸带环境综合试验站
作者单位	1.中国科学院烟台海岸带研究所牟平海岸带环境综合试验站,山东烟台264003; 2.中国科学院海洋大科学中心,山东青岛266071 3.中国科学院大学,北京100049;
推荐引用方式 GB/T 7714	王方晗,王雷,孙婷婷,等. 基于形态学和宏条形码技术的南海西沙群岛浮游动物多样性的比较分析[J]. 应用海洋学学报,2022,41(2):317-327.
APA	王方晗,王雷,孙婷婷,&董志军.(2022).基于形态学和宏条形码技术的南海西沙群岛浮游动物多样性的比较分析.应用海洋学学报,41(2),317-327.
MLA	王方晗,et al."基于形态学和宏条形码技术的南海西沙群岛浮游动物多样性的比较分析".应用海洋学学报 41.2(2022):317-327.