Transcriptomic and physio-biochemical features in rice (Oryza sativa L.) in response to mercury stress

doi:10.1016/j.chemosphere.2022.136612

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院北方资源植物重点实验室

	Transcriptomic and physio-biochemical features in rice (Oryza sativa L.) in response to mercury stress
	Huang, Yingmei 4,5; Li, Fangbai 4; Yi, Jicai 6; Yan, Huili 1; He, Zhenyan 1; Li, Xiaomin 2,3
刊名	CHEMOSPHERE
	2022
卷号	309
关键词	Rice leaf Mercury exposure Chlorophyll Differentially expressed gene Glutathione Reactive oxygen species
ISSN号	0045-6535
DOI	10.1016/j.chemosphere.2022.136612
文献子类	Article
英文摘要	Mercury (Hg) is a toxic and nonessential element for organisms, and its contamination in the environment is a global concern. Previous research has shown that Hg stress may cause severe damage to rice roots; however, the transcriptomic changes in roots and physio-biochemical responses in leaves to different levels of Hg stress are not fully understood. In the present study, rice seedlings were exposed to 20, 80, and 160 mu M HgCl2 for three days in hydroponic experiments. The results showed that the majority of Hg was accumulated in rice roots after Hg exposure, and the 80-and 160-mu M Hg stresses significantly increased the root-to-shoot translocation factors relative to 20-mu M Hg stress, resulting in elevated Hg concentrations in rice shoots. Only the 160-mu M Hg stress significantly inhibited root growth compared with the control, while photosynthesis capacity in leaves was significantly reduced under Hg stress. RNA transcriptome sequencing analyses of the roots showed that common responsive differentially expressed genes were strongly associated with glutathione metabolism, amino acid biosynthesis, and secondary metabolite metabolism, which may play significant roles in Hg accumulation by rice plants. Nine crucial genes identified by protein-protein interaction network analysis may be used as candidate target genes for further investigation of the detoxification mechanism, encoding proteins involved in jasmonic acid synthesis, sugar metabolism, allene oxide synthase, glutathione peroxidase, dismutase, and catalase. Furthermore, physio-biochemical analyses of the leaves indicated that higher production of reactive oxygen species was induced by Hg stress, while glutathione and antioxidant enzymes may play crucial roles in Hg detoxification. Our findings provide transcriptomic and physio-biochemical features of rice roots and shoots, which advance our understanding of the responsive and detoxification mechanisms in rice under different levels of Hg stress.
学科主题	Environmental Sciences
电子版国际标准刊号	1879-1298
出版地	OXFORD
WOS关键词	PHENYLALANINE AMMONIA-LYASE ; INORGANIC MERCURY ; TOXICITY ; CADMIUM ; METHYLMERCURY ; TRANSLOCATION ; GLUTATHIONE ; POLLUTION ; PATHWAY ; ROOTS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000933948900003
资助机构	National Natural Sci- ence Foundation of China [42030702] ; Guangdong Key Research and Development Project [2019B110207002] ; Natural Science Foundation of Guangdong Province [2021A1515110574] ; Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program [2017BT01Z176]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/28752]
专题	中科院北方资源植物重点实验室
作者单位	1.South China Agr Univ, Coll Life Sci, Guangzhou 510642, Peoples R China 2.Chinese Acad Sci, Guangzhou Inst Geochem, Guangzhou 510640, Peoples R China 3.South China Normal Univ, SCNU Environm Res Inst, Guangdong Prov Key Lab Chem Pollut & Environm Safe, Guangzhou 510006, Peoples R China 4.South China Normal Univ, MOE Key Lab Theoret Chem Environm, Guangzhou 510006, Peoples R China 5.Guangdong Acad Sci, Inst Ecoenvironm & Soil Sci, Natl Reg Joint Engn Res Ctr Soil Pollut Control &, Guangdong Key Lab Integrated Agroenvironm Pollut C, Guangzhou 510650, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 7.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Huang, Yingmei,Li, Fangbai,Yi, Jicai,et al. Transcriptomic and physio-biochemical features in rice (Oryza sativa L.) in response to mercury stress[J]. CHEMOSPHERE,2022,309.
APA	Huang, Yingmei,Li, Fangbai,Yi, Jicai,Yan, Huili,He, Zhenyan,&Li, Xiaomin.(2022).Transcriptomic and physio-biochemical features in rice (Oryza sativa L.) in response to mercury stress.CHEMOSPHERE,309.
MLA	Huang, Yingmei,et al."Transcriptomic and physio-biochemical features in rice (Oryza sativa L.) in response to mercury stress".CHEMOSPHERE 309(2022).