OsGLYI3, a glyoxalase gene expressed in rice seed, contributes to seed longevity and salt stress tolerance

doi:10.1016/j.plaphy.2022.04.028

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	OsGLYI3, a glyoxalase gene expressed in rice seed, contributes to seed longevity and salt stress tolerance
	Liu, Shengjie 1; Liu, Wenhua 1; Lai, Jianyun 1; Liu, Qinjian 1; Zhang, Wenhu 1; Chen, Zhongjian 1; Gao, Jiadong 1; Song, Songquan 3; Liu, Jun 1; Xiao, Yinghui
刊名	PLANT PHYSIOLOGY AND BIOCHEMISTRY
	2022
卷号	183 页码:85-95
关键词	Glyoxalase I Methylglyoxal Stress tolerance Seed longevity Rice(Oryza sativa L )
ISSN号	0981-9428
DOI	10.1016/j.plaphy.2022.04.028
文献子类	Article
英文摘要	The glyoxalase pathway plays a vital role in the chemical detoxification of methylglyoxal (MG) in biological systems. Our previous study suggested that OsGLYI3 may be effective in seed natural aging. In this study, the rice OsGLYI3 gene was cloned and characterized as specifically expressed in the seed. The accelerated aging (AA) treatment results indicated significant roles of OsGLYI3 in seed longevity and vigor, as the seeds of the transgenic lines with overexpressed and knocked-out OsGLYI3 exhibited higher and lower germination, respectively. The AA treatment also increased the superoxide dismutase (SOD) activity in the overexpressed transgenic seeds compared to the wild-type seeds yet lowered the SOD activity in the CRISPR/Cas9-derived transgenic rice lines. Rice OsGLYI3 was markedly upregulated in response to NaCl induced stress conditions. Compared to wild-type plants, overexpressed transgenic rice lines exhibited increased GLYI activity, decreased MG levels and improved salt stress tolerance, while CRISPR/Cas9 knockout transgenic rice lines showed decreased glyoxalase I activity, increased MG levels, and greater sensitivity to stress treatments with NaCl. Collectively, our results confirmed for the first time that OsGLYI3 is specifically expressed in rice seeds and contributes to seed longevity and salt stress tolerance.
学科主题	Plant Sciences
电子版国际标准刊号	1873-2690
出版地	ISSY-LES-MOULINEAUX
WOS关键词	ORYZA-SATIVA ; I GENE ; METHYLGLYOXAL ; OVEREXPRESSION ; PROTEIN ; IDENTIFICATION ; DETOXIFICATION ; DETERIORATION ; GERMINATION ; DORMANCY
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
WOS记录号	WOS:000802221400003
资助机构	National Natural Science Foundation, China [31871716] ; Science and Technology Program of Guangdong Province, China [2020B121201008, 2020B020209003, 2018B020202004] ; Science and Technology Program of Guangzhou [201909020001, 201807010114] ; Guangdong regional joint fund for basic and applied basic research-key project [2019B1515120061]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/28678]
专题	中科院北方资源植物重点实验室
作者单位	1.Hunan Agr Univ, Coll Agron, Changsha 410128, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 3.Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangdong Key Lab Crop Germplasm Resources Preserv, Guangzhou 510640, Peoples R China
推荐引用方式 GB/T 7714	Liu, Shengjie,Liu, Wenhua,Lai, Jianyun,et al. OsGLYI3, a glyoxalase gene expressed in rice seed, contributes to seed longevity and salt stress tolerance[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY,2022,183:85-95.
APA	Liu, Shengjie.,Liu, Wenhua.,Lai, Jianyun.,Liu, Qinjian.,Zhang, Wenhu.,...&Xiao, Yinghui.(2022).OsGLYI3, a glyoxalase gene expressed in rice seed, contributes to seed longevity and salt stress tolerance.PLANT PHYSIOLOGY AND BIOCHEMISTRY,183,85-95.
MLA	Liu, Shengjie,et al."OsGLYI3, a glyoxalase gene expressed in rice seed, contributes to seed longevity and salt stress tolerance".PLANT PHYSIOLOGY AND BIOCHEMISTRY 183(2022):85-95.