Crystal Structures of Arabidopsis thaliana GDP-D-Mannose Pyrophosphorylase VITAMIN C DEFECTIVE 1

doi:10.3389/fpls.2022.899738

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院光生物学重点实验室

	Crystal Structures of Arabidopsis thaliana GDP-D-Mannose Pyrophosphorylase VITAMIN C DEFECTIVE 1
	Zhang, Chi ; Zhao, Shun 2,4; Li, Yu-Shuai ; He, Chao 1; Wang, Xiao 1; Liu, Lin 1
刊名	FRONTIERS IN PLANT SCIENCE
	2022
卷号	13
关键词	ascorbic acid nucleotide sugar guanylyltransferase crystallography oligomerization
ISSN号	1664-462X
DOI	10.3389/fpls.2022.899738
文献子类	Article
英文摘要	Plant GDP-D-mannose pyrophosphorylase (GMPase) catalyzes a committed step in ascorbic acid biosynthesis pathway. Arabidopsis thaliana VTC1 is the first genetically characterized plant GMPase and has unique properties when compared with bacterial and animal homologs. Here we present the crystal structures of VTC1 in the unliganded and product-bound states at resolutions of 2.8 and 3.0 angstrom, respectively. VTC1 dimerizes in a same way like other known GMPases, but dodecamerizes in a previously unobserved arrangement. The interactions to GDP-D-mannose and inorganic pyrophosphate are revealed by the product-bound VTC1 structure. An in vitro GMPase activity assay confirms the regulatory role of the C-terminal left-handed beta-helix domain, and structural analyses suggest the models of VTC1 hetero-complex with its interacting proteins. The structural information advances our insights into the different mechanisms involved in VTC1 regulation.
学科主题	Plant Sciences
出版地	LAUSANNE
WOS关键词	L-ASCORBIC-ACID ; STRUCTURE REFINEMENT ; BIOSYNTHESIS ; METABOLISM ; DEFICIENT ; MUTANT ; MODEL ; CYTOKINESIS ; AMMONIUM ; MUTATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000807112400001
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/28997]
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing, Peoples R China 2.Anhui Univ, Anhui Key Lab Modern Biomfg, Hefei, Peoples R China 3.Mem Sloan Kettering Canc Ctr, Struct Biol Program, New York, NY USA 4.Anhui Univ, Sch Life Sci, Hefei, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Chi,Zhao, Shun,Li, Yu-Shuai,et al. Crystal Structures of Arabidopsis thaliana GDP-D-Mannose Pyrophosphorylase VITAMIN C DEFECTIVE 1[J]. FRONTIERS IN PLANT SCIENCE,2022,13.
APA	Zhang, Chi,Zhao, Shun,Li, Yu-Shuai,He, Chao,Wang, Xiao,&Liu, Lin.(2022).Crystal Structures of Arabidopsis thaliana GDP-D-Mannose Pyrophosphorylase VITAMIN C DEFECTIVE 1.FRONTIERS IN PLANT SCIENCE,13.
MLA	Zhang, Chi,et al."Crystal Structures of Arabidopsis thaliana GDP-D-Mannose Pyrophosphorylase VITAMIN C DEFECTIVE 1".FRONTIERS IN PLANT SCIENCE 13(2022).