The CDC48 complex mediates ubiquitin-dependent degradation of intra-chloroplast proteins in plants

doi:10.1016/j.celrep.2022.110664

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	The CDC48 complex mediates ubiquitin-dependent degradation of intra-chloroplast proteins in plants
	Li, Jialong ; Yuan, Jiarui 1; Li, Yuhong 1; Sun, Huilun 1; Ma, Tingting ; Huai, Junling ; Yang, Wenqiang 1; Zhang, Wenhao 1,3; Lin, Rongcheng 1
刊名	CELL REPORTS
	2022
卷号	39 期号:2
ISSN号	2211-1247
DOI	10.1016/j.celrep.2022.110664
文献子类	Article
英文摘要	Chloroplasts are the site of numerous biochemical reactions including photosynthesis, but they also produce reactive oxygen species (ROS) that negatively affect chloroplast integrity. The chaperone-like CDC48 com-plex plays critical roles in ubiquitin-dependent protein degradation in yeast and mammals, but its function in plants is largely unknown. Here, we show that defects in CDC48A and its cofactors UFD1 and NPL4 lead to the accumulation of ubiquitinated chloroplast proteins in Arabidopsis thaliana. We reveal that two plastid genome-encoded proteins, RbcL and AtpB, associate with the CDC48 complex. Strikingly, RbcL and AtpB are ubiquitinated and degraded by the 26S proteasome pathway upon ROS stress, and these processes are impaired by defects of the CDC48 complex. Functional analysis demonstrates that the CDC48 complex is required for plant tolerance to ROS. This study reveals a role for the plant CDC48 complex in modulating ubiquitin-dependent degradation of intra-chloroplast proteins in response to oxidative stress.
学科主题	Cell Biology
出版地	CAMBRIDGE
WOS关键词	E3 LIGASE ATCHIP ; QUALITY-CONTROL ; REACTIVE OXYGEN ; LYSINE UBIQUITINATION ; PROTEASOME SYSTEM ; FTSH PROTEASE ; RETROTRANSLOCATION ; BIOGENESIS ; SUBSTRATE ; REVEALS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	CELL PRESS
WOS记录号	WOS:000793180100001
资助机构	National Key Research and Development Program of China [2020YFA0907600, 2019YFA0904600] ; National Natural Science Foundation of China [32030009] ; Strategic Priority Research Program of the Chinese Academy of Sciences [XDB27030205]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/28758]
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Jialong,Yuan, Jiarui,Li, Yuhong,et al. The CDC48 complex mediates ubiquitin-dependent degradation of intra-chloroplast proteins in plants[J]. CELL REPORTS,2022,39(2).
APA	Li, Jialong.,Yuan, Jiarui.,Li, Yuhong.,Sun, Huilun.,Ma, Tingting.,...&Lin, Rongcheng.(2022).The CDC48 complex mediates ubiquitin-dependent degradation of intra-chloroplast proteins in plants.CELL REPORTS,39(2).
MLA	Li, Jialong,et al."The CDC48 complex mediates ubiquitin-dependent degradation of intra-chloroplast proteins in plants".CELL REPORTS 39.2(2022).