The RNA helicase UAP56 and the E3 ubiquitin ligase COP1 coordinately regulate alternative splicing to repress photomorphogenesis in Arabidopsis

doi:10.1093/plcell/koac235

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	The RNA helicase UAP56 and the E3 ubiquitin ligase COP1 coordinately regulate alternative splicing to repress photomorphogenesis in Arabidopsis
	Li, Yang 1; Du, Yanxin 1; Huai, Junling ; Jing, Yanjun ; Lin, Rongcheng 1
刊名	PLANT CELL
	2022
卷号	34 期号:11 页码:4191-4212
ISSN号	1040-4651
DOI	10.1093/plcell/koac235
文献子类	Article
英文摘要	Light is a key environmental signal that regulates plant growth and development. While posttranscriptional regulatory mechanisms of gene expression include alternative splicing (AS) of pre-messenger RNA (mRNA) in both plants and animals, how light signaling affects AS in plants is largely unknown. Here, we identify DExD/H RNA helicase U2AF65-associated protein (UAP56) as a negative regulator of photomorphogenesis in Arabidopsis thaliana. UAP56 is encoded by the homologs UAP56a and UAP56b. Knockdown of UAP56 led to enhanced photomorphogenic responses and diverse developmental defects during vegetative and reproductive growth. UAP56 physically interacts with the central light signaling repressor constitutive photomorphogenic 1 (COP1) and U2AF65. Global transcriptome analysis revealed that UAP56 and COP1 co-regulate the transcription of a subset of genes. Furthermore, deep RNA-sequencing analysis showed that UAP56 and COP1 control pre-mRNA AS in both overlapping and distinct manners. Ribonucleic acid immunoprecipitation assays showed that UAP56 and COP1 bind to common small nuclear RNAs and mRNAs of downstream targets. Our study reveals that both UAP56 and COP1 function as splicing factors that coordinately regulate AS during light-regulated plant growth and development. The RNA helicase UAP56 and the E3 ubiquitin ligase COP1 function as splicing factors to coordinately regulate alternative splicing during light-regulated plant growth and development.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
电子版国际标准刊号	1532-298X
出版地	CARY
WOS关键词	PROTEIN INTERACTIONS ; LIGHT ; DEETIOLATION ; ROLES ; SPLICEOSOME ; INTERACTS ; PROMOTES ; PLANTS ; HY5
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	OXFORD UNIV PRESS INC
WOS记录号	WOS:000840130000001
资助机构	National Key R&D Program of China [2020YFA0907601] ; National Natural Science Foundation of China [32030009] ; Strategic Priority Research Program of the Chinese Academy of Sciences [XDB27030205]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/28614]
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Yang,Du, Yanxin,Huai, Junling,et al. The RNA helicase UAP56 and the E3 ubiquitin ligase COP1 coordinately regulate alternative splicing to repress photomorphogenesis in Arabidopsis[J]. PLANT CELL,2022,34(11):4191-4212.
APA	Li, Yang,Du, Yanxin,Huai, Junling,Jing, Yanjun,&Lin, Rongcheng.(2022).The RNA helicase UAP56 and the E3 ubiquitin ligase COP1 coordinately regulate alternative splicing to repress photomorphogenesis in Arabidopsis.PLANT CELL,34(11),4191-4212.
MLA	Li, Yang,et al."The RNA helicase UAP56 and the E3 ubiquitin ligase COP1 coordinately regulate alternative splicing to repress photomorphogenesis in Arabidopsis".PLANT CELL 34.11(2022):4191-4212.