Cold-induced calreticulin OsCRT3 conformational changes promote OsCIPK7 binding and temperature sensing in rice

doi:10.15252/embj.2021110518

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	Cold-induced calreticulin OsCRT3 conformational changes promote OsCIPK7 binding and temperature sensing in rice
	Guo, Xiaoyu 4; Zhang, Dajian 4; Wang, Zhongliang 4; Xu, Shujuan 4; Batistic, Oliver 3; Steinhorst, Leonie 3; Li, Hao 6; Weng, Yuxiang 6; Ren, Dongtao 1; Kudla, Jorg 3
刊名	EMBO JOURNAL
	2023
卷号	42 期号:1
关键词	Calcium signal Calreticulin Cold stress OsCIPK7 OsCRT3
ISSN号	0261-4189
DOI	10.15252/embj.2021110518
文献子类	Article
英文摘要	Unusually low temperatures caused by global climate change adversely affect rice production. Sensing cold to trigger signal network is a key base for improvement of chilling tolerance trait. Here, we report that Oryza sativa Calreticulin 3 (OsCRT3) localized at the endoplasmic reticulum (ER) exhibits conformational changes under cold stress, thereby enhancing its interaction with CBL-interacting protein kinase 7 (OsCIPK7) to sense cold. Phenotypic analyses of OsCRT3 knock-out mutants and transgenic overexpression lines demonstrate that OsCRT3 is a positive regulator in chilling tolerance. OsCRT3 localizes at the ER and mediates increases in cytosolic calcium levels under cold stress. Notably, cold stress triggers secondary structural changes of OsCRT3 and enhances its binding affinity with OsCIPK7, which finally boosts its kinase activity. Moreover, Calcineurin B-like protein 7 (OsCBL7) and OsCBL8 interact with OsCIPK7 specifically on the plasma membrane. Taken together, our results thus identify a cold-sensing mechanism that simultaneously conveys cold-induced protein conformational change, enhances kinase activity, and Ca2+ signal generation to facilitate chilling tolerance in rice.
学科主题	Biochemistry & Molecular Biology ; Cell Biology
电子版国际标准刊号	1460-2075
出版地	HOBOKEN
WOS关键词	DNA INSERTIONAL MUTAGENESIS ; PROTEIN-KINASE SOS2 ; ENDOPLASMIC-RETICULUM ; STRESS RESPONSES ; CALCIUM SENSORS ; PLANT-CELLS ; CALCINEURIN ; DOMAIN ; SALT ; CA2+
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	WILEY
WOS记录号	WOS:000879368400001
资助机构	Basic Science Center Project of National Natural Science Foundation of China [31788103] ; National Key Research and Development Program [2020YFE0202300-06] ; Special Research Assistant Project of Chinese Academy of Sciences [2021000017] ; China Postdoctoral Science Foundation [2020M670519] ; Deutsche Forschungsgemeinschaft (DFG) [INST 211/565-1 FUGG, INST211/853-1 FUGG]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/29148]
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Phys, Lab Soft Matter Phys, Beijing, Peoples R China 2.Chinese Acad Sci, Innovat Acad Seed Design, Beijing, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China 5.China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing, Peoples R China 6.Westfalische Wilhelms Univ, Inst Biol & Biotechnol Pflanzen, Munster, Germany
推荐引用方式 GB/T 7714	Guo, Xiaoyu,Zhang, Dajian,Wang, Zhongliang,et al. Cold-induced calreticulin OsCRT3 conformational changes promote OsCIPK7 binding and temperature sensing in rice[J]. EMBO JOURNAL,2023,42(1).
APA	Guo, Xiaoyu.,Zhang, Dajian.,Wang, Zhongliang.,Xu, Shujuan.,Batistic, Oliver.,...&Chong, Kang.(2023).Cold-induced calreticulin OsCRT3 conformational changes promote OsCIPK7 binding and temperature sensing in rice.EMBO JOURNAL,42(1).
MLA	Guo, Xiaoyu,et al."Cold-induced calreticulin OsCRT3 conformational changes promote OsCIPK7 binding and temperature sensing in rice".EMBO JOURNAL 42.1(2023).