A multiomic study uncovers a bZIP23-PER1A-mediated detoxification pathway to enhance seed vigor in rice

doi:10.1073/pnas.2026355119

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	A multiomic study uncovers a bZIP23-PER1A-mediated detoxification pathway to enhance seed vigor in rice
	Wang, Wei-Qing ; Xu, Ding-Yi ; Sui, Ya-Ping ; Ding, Xiao-Hui ; Song, Xian-Jun
刊名	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
	2022
卷号	119 期号:9
关键词	seed vigor ABA signaling reactive oxygen species
ISSN号	0027-8424
DOI	10.1073/pnas.2026355119
文献子类	Article
英文摘要	Seed vigor in crops is important in terms of improving grain quality and germplasm conservation; however, little is known about its regulatory mechanisms through the encoded proteome and gene network. Comparative analyses of transcriptome (RNA sequencing [RNA-seq]) and broadly targeted metabolic profiling of two subspecific rice cultivars with distinct seed vigor during accelerated aging revealed various biological pathways and metabolic processes as key influences explaining trait differences. RNA-seq coexpression regulatory network analyses identified several transcription factors, including bZIP23 and bZIP42, that act as nodes in the gene network. Importantly, transgenic seeds of overexpression of bZIP23 enhanced seed vigor, whereas its gene knockout reduced seed vigor, suggesting that the protein it encodes functions as a positive regulator. Similarly, overexpression and knockout of PER1A that encodes a key player in the detoxification pathway enhanced and decreased seed vigor, respectively. We further demonstrated a direct interaction of the PER1A promoter with bZIP23 in seeds, which activates the expression of PER1A, and the genetic evidence suggested that bZIP23 most likely functions in a common pathway with and acts upstream of PER1A to modulate seed vigor. In addition, the control of seed vigor by the bZIP23-PER1A module was connected with that of the abscisic acid signaling pathway. Collectively, we revealed the genetic architecture of variation in seed vigor and uncovered the bZIP23-PER1A-mediated detoxification pathway that enhances the trait in rice.
学科主题	Multidisciplinary Sciences
电子版国际标准刊号	1091-6490
出版地	WASHINGTON
WOS关键词	TRANSCRIPTION FACTOR FAMILY ; INDUCED ANTIOXIDANT DEFENSE ; IMPROVES DROUGHT TOLERANCE ; ZINC-FINGER PROTEIN ; PEROXIREDOXIN ANTIOXIDANT ; POSITIVE REGULATOR ; OXIDATIVE STRESS ; KEY PLAYER ; ACID ; EXPRESSION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	NATL ACAD SCIENCES
WOS记录号	WOS:000766706200007
资助机构	CAS [XDA24010101-2] ; National Key Research and Development Program of China [2016YFD0100402] ; National Natural Science Foundation of China [91735302, 91435113, 31471466, 31770357] ; Youth Innovation Promotion Association, CAS
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/28734]
专题	中科院植物分子生理学重点实验室
作者单位	1.Xu, Ding-Yi 2.[Wang, Wei-Qing 3.[Sui, Ya-Ping 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 5.Ding, Xiao-Hui 6.Sui, Ya-Ping 7.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100101, Peoples R China 8.Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Wei-Qing,Xu, Ding-Yi,Sui, Ya-Ping,et al. A multiomic study uncovers a bZIP23-PER1A-mediated detoxification pathway to enhance seed vigor in rice[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2022,119(9).
APA	Wang, Wei-Qing,Xu, Ding-Yi,Sui, Ya-Ping,Ding, Xiao-Hui,&Song, Xian-Jun.(2022).A multiomic study uncovers a bZIP23-PER1A-mediated detoxification pathway to enhance seed vigor in rice.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,119(9).
MLA	Wang, Wei-Qing,et al."A multiomic study uncovers a bZIP23-PER1A-mediated detoxification pathway to enhance seed vigor in rice".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 119.9(2022).