GhBZR3 suppresses cotton fiber elongation by inhibiting very-long-chain fatty acid biosynthesis

doi:10.1111/tpj.15852

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院植物分子生理学重点实验室

	GhBZR3 suppresses cotton fiber elongation by inhibiting very-long-chain fatty acid biosynthesis
	Shi, Zemin 1; Chen, Xia 1; Xue, Huidan 2,5; Jia, Tingting 3; Meng, Funing 1; Liu, Yunfei 1; Luo, Xiaomin ; Xiao, Guanghui 3; Zhu, Shengwei
刊名	PLANT JOURNAL
	2022
卷号	111 期号:3 页码:785-799
关键词	brassinosteroids GhBZR3 VLCFA biosynthesis cotton fiber
ISSN号	0960-7412
DOI	10.1111/tpj.15852
文献子类	Article
英文摘要	The BRASSINAZOLE-RESISTANT (BZR) transcription factor is a core component of brassinosteroid (BR) signaling and is involved in the development of many plant species. BR is essential for the initiation and elongation of cotton fibers. However, the mechanism of BR-regulating fiber development and the function of BZR is poorly understood in Gossypium hirsutum L. (cotton). Here, we identified a BZR family transcription factor protein referred to as GhBZR3 in cotton. Overexpression of GhBZR3 in Arabidopsis caused shorter root hair length, hypocotyl length, and hypocotyl cell length, indicating that GhBZR3 negatively regulates cell elongation. Pathway enrichment analysis from VIGS-GhBZR3 cotton plants found that fatty acid metabolism and degradation might be the regulatory pathway that is primarily controlled by GhBZR3. Silencing GhBZR3 expression in cotton resulted in taller plant height as well as longer fibers. The very-long-chain fatty acid (VLCFA) content was also significantly increased in silenced GhBZR3 plants compared with the wild type. The GhKCS13 promoter, a key gene for VLCFA biosynthesis, contains two GhBZR3 binding sites. The results of yeast one-hybrid, electrophoretic mobility shift, and luciferase assays revealed that GhBZR3 directly interacted with the GhKCS13 promoter to suppress gene expression. Taken together, these results indicate that GhBZR3 negatively regulates cotton fiber development by reducing VLCFA biosynthesis. This study not only deepens our understanding of GhBZR3 function in cotton fiber development, but also highlights the potential of improving cotton fiber length and plant growth using GhBZR3 and its related genes in future cotton breeding programs.
学科主题	Plant Sciences
电子版国际标准刊号	1365-313X
出版地	HOBOKEN
WOS关键词	CELL ELONGATION ; TRANSCRIPTION FACTOR ; GENE-EXPRESSION ; 14-3-3 PROTEINS ; ABIOTIC STRESS ; ARABIDOPSIS ; NETWORK ; GROWTH ; FAMILY ; BRASSINOSTEROIDS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	WILEY
WOS记录号	WOS:000812628600001
资助机构	National Key Research and Development Program [2020YFA0907600, 2018YFD0100401] ; National Research and Development Project of Transgenic Crops of China [2018ZX0800921B] ; National Natural Science Foundation of China [32070549] ; Young Elite Scientists Sponsorship Program by CAST [2019-2021QNRC001] ; State Key Laboratory of Cotton Biology Open Fund [CB2016A01] ; China Postdoctoral Science Foundation [2020M683549] ; Hainan Yazhou Bay Seed Laboratory [B21HJ2005]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/28667]
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Shaanxi Univ Sci & Technol, Sch Food & Biol Engn, Xian 710021, Peoples R China 3.Northwestern Polytech Univ, Sch Ecol & Environm, Xian 710012, Peoples R China 4.Shaanxi Normal Univ, Coll Life Sci, Xian 710062, Peoples R China 5.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Shi, Zemin,Chen, Xia,Xue, Huidan,et al. GhBZR3 suppresses cotton fiber elongation by inhibiting very-long-chain fatty acid biosynthesis[J]. PLANT JOURNAL,2022,111(3):785-799.
APA	Shi, Zemin.,Chen, Xia.,Xue, Huidan.,Jia, Tingting.,Meng, Funing.,...&Zhu, Shengwei.(2022).GhBZR3 suppresses cotton fiber elongation by inhibiting very-long-chain fatty acid biosynthesis.PLANT JOURNAL,111(3),785-799.
MLA	Shi, Zemin,et al."GhBZR3 suppresses cotton fiber elongation by inhibiting very-long-chain fatty acid biosynthesis".PLANT JOURNAL 111.3(2022):785-799.