A group of CLE peptides regulates de novo shoot regeneration in Arabidopsis thaliana

doi:10.1111/nph.18291

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	A group of CLE peptides regulates de novo shoot regeneration in Arabidopsis thaliana
	Kang, Jingke ; Wang, Xuening ; Ishida, Takashi 5; Grienenberger, Etienne ; Zheng, Qian ; Wang, Jing ; Zhang, Yonghong 4; Chen, Wenqiang ; Chen, Mengmeng ; Song, Xiu-Fen
刊名	NEW PHYTOLOGIST
	2022
卷号	235 期号:6 页码:2300-2312
关键词	Arabidopsis thaliana BAM1 CLE peptide CLV1 shoot regeneration
ISSN号	0028-646X
DOI	10.1111/nph.18291
文献子类	Article
英文摘要	Known for their regulatory roles in stem cell homeostasis, CLAVATA3/ESR-RELATED (CLE) peptides also function as mediators of external stimuli such as hormones. De novo shoot regeneration, representing the remarkable plant cellular plasticity, involves reconstitution of stem cells under control of stem-cell regulators. Yet whether and how stem cell-regulating CLE peptides are implicated in plant regeneration remains unknown. By CRISPR/Cas9-induced loss-of-function studies, peptide application, precursor overexpression, and expression analyses, the role of CLE1-CLE7 peptides and their receptors in de novo shoot regeneration was studied in Arabidopsis thaliana. CLE1-CLE7 are induced by callus-induction medium and dynamically expressed in pluripotent callus. Exogenously-applied CLE1-CLE7 peptides or precursor overexpression effectively leads to shoot regeneration suppression, whereas their simultaneous mutation results in enhanced regenerative capacity, demonstrating that CLE1-CLE7 peptides redundantly function as negative regulators of de novo shoot regeneration. CLE1-CLE7-mediated shoot regeneration suppression is impaired in loss-of-function mutants of callus-expressed CLAVATA1 (CLV1) and BARELY ANY MERISTEM1 (BAM1) genes, indicating that CLV1/BAM1 are required for CLE1-CLE7-mediated shoot regeneration signaling. CLE1-CLE7 signaling resulted in transcriptional repression of WUSCHEL (WUS), a stem cell-promoting transcription factor known as a principal regulator of plant regeneration. Our results indicate that functionally-redundant CLE1-CLE7 peptides genetically act through CLV1/BAM1 receptors and repress WUS expression to modulate shoot-regeneration capacity, establishing the mechanistic basis for CLE1-CLE7-mediated shoot regeneration and a novel role for CLE peptides in hormone-dependent developmental plasticity.
学科主题	Plant Sciences
电子版国际标准刊号	1469-8137
出版地	HOBOKEN
WOS关键词	OF-FUNCTION PHENOTYPES ; STEM-CELL FATE ; SIGNALING PATHWAY ; GENES ; ROOT ; WUSCHEL ; MAINTENANCE ; DISSECTION ; SYSTEMS ; CLAVATA
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	WILEY
WOS记录号	WOS:000812979300001
资助机构	National Natural Science Foundation of China [31771556, 31271575, 31200902, 31701294, 32000609] ; Natural Science Foundation of Shaanxi for Distinguished Young Scholars [2020JC-29] ; United States Department of Agriculture [CRIS 2030-21000-048-00D] ; 100-Talent Program of Shaanxi Province ; Fundamental Research Funds for the Central Universities [GK202002008] ; Key Project at Central Government Level: The ability establishment of sustainable use for valuable Chinese medicine resources [2060302]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/28552]
专题	中科院植物分子生理学重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 3.Hubei Univ Med, Acad Biomed Res, Sch Basic Med, Lab Med Plant,Hubei Key Lab Wudang Local Chinese, Shiyan 442000, Peoples R China 4.Grienenberger, Etienne; Fletcher, Jennifer C.] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA 5.Shaanxi Normal Univ, Coll Life Sci, Natl Engn Lab Endangered Med Resource Dev Northwe, Key Lab Med Resources & Nat Pharmaceut Chem Minis, Xian 710119, Peoples R China 6.Kumamoto Univ, Int Res Org Adv Sci & Technol IROAST, Kumamoto 8608555, Japan 7.Grienenberger, Etienne; Fletcher, Jennifer C.] USDA ARS UC Berkeley, Plant Gene Express Ctr, Albany, CA 94710 USA 8.Int Res Ctr Agr & Environm Biol IRCAEB, Chuo Ku, 2-39-1 Kurokami, Kumamoto 8608555, Japan 9.Henan Univ, Sch Life Sci, Key Lab Plant Stress Biol, Kaifeng 475004, Peoples R China
推荐引用方式 GB/T 7714	Kang, Jingke,Wang, Xuening,Ishida, Takashi,et al. A group of CLE peptides regulates de novo shoot regeneration in Arabidopsis thaliana[J]. NEW PHYTOLOGIST,2022,235(6):2300-2312.
APA	Kang, Jingke.,Wang, Xuening.,Ishida, Takashi.,Grienenberger, Etienne.,Zheng, Qian.,...&Wang, Guodong.(2022).A group of CLE peptides regulates de novo shoot regeneration in Arabidopsis thaliana.NEW PHYTOLOGIST,235(6),2300-2312.
MLA	Kang, Jingke,et al."A group of CLE peptides regulates de novo shoot regeneration in Arabidopsis thaliana".NEW PHYTOLOGIST 235.6(2022):2300-2312.