Multiplexed Massively Parallel Sequencing of Plastomes Provides Insights Into the Genetic Diversity, Population Structure, and Phylogeography of Wild and Cultivated Coptis chinensis

doi:10.3389/fpls.2022.923600

CORC > 植物研究所 > 中国科学院植物研究所 > 系统与进化植物学国家重点实验室

	Multiplexed Massively Parallel Sequencing of Plastomes Provides Insights Into the Genetic Diversity, Population Structure, and Phylogeography of Wild and Cultivated Coptis chinensis
	Wang, Yiheng ; Sun, Jiahui ; Zhao, Zhenyu ; Xu, Chao 1; Qiao, Ping 3; Wang, Sheng ; Wang, Mengli ; Xu, Zegang 4; Yuan, Qingjun ; Guo, Lanping
刊名	FRONTIERS IN PLANT SCIENCE
	2022
卷号	13
关键词	Coptis chinensis wild and cultivated genetic diversity phylogeography population structure massively parallel sequencing plastome
ISSN号	1664-462X
DOI	10.3389/fpls.2022.923600
文献子类	Article
英文摘要	Root rot has been a major problem for cultivated populations of Coptis chinensis var. chinensis in recent years. C. chinensis var. brevisepala, the closest wild relative of C. chinensis var. chinensis, has a scattered distribution across southwestern China and is an important wild resource. Genetic diversity is associated with greater evolutionary potential and resilience of species or populations and is important for the breeding and conservation of species. Here, we conducted multiplexed massively parallel sequencing of the plastomes of 227 accessions of wild and cultivated C. chinensis using 111 marker pairs to study patterns of genetic diversity, population structure, and phylogeography among wild and cultivated C. chinensis populations. Wild and cultivated resources diverged approximately 2.83 Mya. The cultivated resources experienced a severe genetic bottleneck and possess highly mixed germplasm. However, high genetic diversity has been retained in the wild resources, and subpopulations in different locations differed in genotype composition. The significant divergence in the genetic diversity of wild and cultivated resources indicates that they require different conservation strategies. Wild resources require in situ conservation strategies aiming to expand population sizes while maintaining levels of genetic diversity; by contrast, germplasm resource nurseries with genotypes of cultivated resources and planned distribution measures are needed for the conservation of cultivated resources to prevent cultivated populations from undergoing severe genetic bottlenecks. The results of this study provide comprehensive insights into the genetic diversity, population structure, and phylogeography of C. chinensis and will facilitate future breeding and conservation efforts.
学科主题	Plant Sciences
出版地	LAUSANNE
WOS关键词	DOMESTICATION ; ACCESSIONS ; SOFTWARE ; PROGRAM
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000844607600001
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/28882]
专题	系统与进化植物学国家重点实验室
作者单位	1.China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, Beijing, Peoples R China 2.Lichuan Jianzhuxi Huanglian Cooperat, Lichuan, Peoples R China 3.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing, Peoples R China 4.Jiangxi Univ Tradit Chinese Med, Academician Workstat, Nanchang, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yiheng,Sun, Jiahui,Zhao, Zhenyu,et al. Multiplexed Massively Parallel Sequencing of Plastomes Provides Insights Into the Genetic Diversity, Population Structure, and Phylogeography of Wild and Cultivated Coptis chinensis[J]. FRONTIERS IN PLANT SCIENCE,2022,13.
APA	Wang, Yiheng.,Sun, Jiahui.,Zhao, Zhenyu.,Xu, Chao.,Qiao, Ping.,...&Huang, Luqi.(2022).Multiplexed Massively Parallel Sequencing of Plastomes Provides Insights Into the Genetic Diversity, Population Structure, and Phylogeography of Wild and Cultivated Coptis chinensis.FRONTIERS IN PLANT SCIENCE,13.
MLA	Wang, Yiheng,et al."Multiplexed Massively Parallel Sequencing of Plastomes Provides Insights Into the Genetic Diversity, Population Structure, and Phylogeography of Wild and Cultivated Coptis chinensis".FRONTIERS IN PLANT SCIENCE 13(2022).