Molecular Characterization of Bacterial Isolates from Soil Samples and Evaluation of their Antibacterial Potential against MDRS

doi:10.3390/molecules27196281

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	Molecular Characterization of Bacterial Isolates from Soil Samples and Evaluation of their Antibacterial Potential against MDRS
	Sadiqi, Shahida ; Hamza, Muhammad 4; Ali, Farooq ; Alam, Sadia 4; Shakeela, Qismat 3; Ahmed, Shehzad ; Ayaz, Asma 2; Ali, Sajid 6; Saqib, Saddam 5,7; Ullah, Fazal 8
刊名	MOLECULES
	2022
卷号	27 期号:19
关键词	multidrug-resistant strains antimicrobial activity secondary metabolites Bacillus soil
DOI	10.3390/molecules27196281
文献子类	Article
英文摘要	Some soil microbes, with their diverse inhabitance, biologically active metabolites, and endospore formation, gave them characteristic predominance and recognition among other microbial communities. The present study collected ten soil samples from green land, agricultural and marshy soil sites of Khyber Pakhtunkhwa, Pakistan. After culturing on described media, the bacterial isolates were identified through phenotypic, biochemical and phylogenetic analysis. Our phylogenetic analysis revealed three bacterial isolates, A6S7, A1S6, and A1S10, showing 99% nucleotides sequence similarity with Brevibacillus formosus, Bacillus Subtilis and Paenibacillus dendritiformis. The crude extract was prepared from bacterial isolates to assess the anti-bacterial potential against various targeted multidrug-resistant strains (MDRS), including Acinetobacter baumannii (ATCC 19606), Methicillin-resistant Staphylococcus aureus (MRSA) (BAA-1683), Klebsiella pneumoniae (ATCC 13883), Pseudomonas aeruginosa (BAA-2108), Staphylococcus aureus (ATCC 292013), Escherichia coli (ATCC25922) and Salmonella typhi (ATCC 14028). Our analysis revealed that all bacterial extracts possess activity against Gram-negative and Gram-positive bacteria at a concentration of 5 mg/mL, efficiently restricting the growth of E. coli compared with positive control ciprofloxacin. The study concluded that the identified species have the potential to produce antimicrobial compounds which can be used to control different microbial infections, especially MDRS. Moreover, the analysis of the bacterial extracts through GC-MS indicated the presence of different antimicrobial compounds such as propanoic acid, oxalic acid, phenol and hexadecanoic acid.
学科主题	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
电子版国际标准刊号	1420-3049
出版地	BASEL
WOS关键词	BACILLUS ; RESISTANCE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	MDPI
WOS记录号	WOS:000867203900001
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/28785]
专题	系统与进化植物学国家重点实验室
作者单位	1.Yeungnam Univ, Dept Life Sci, Gyongsan 38541, South Korea 2.Univ Sci & Technol, Fac Hlth Sci, Dept Microbiol, Abbottabad 22020, KP, Pakistan 3.Univ Haripur, Fac Biol & Biomed Sci, Dept Microbiol, Haripur 21120, KP, Pakistan 4.Hazara Univ Mansehra, Fac Biol & Hlth Sci, Dept Microbiol, Mansehra 21300, KP, Pakistan 5.Yeungnam Univ, Dept Hort & Life Sci, Gyongsan 38541, South Korea 6.Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430062, Peoples R China 7.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 8.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 9.Lanzhou Univ, Sch Life Sci, State Key Lab Grassland Agroecosyst, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Sadiqi, Shahida,Hamza, Muhammad,Ali, Farooq,et al. Molecular Characterization of Bacterial Isolates from Soil Samples and Evaluation of their Antibacterial Potential against MDRS[J]. MOLECULES,2022,27(19).
APA	Sadiqi, Shahida.,Hamza, Muhammad.,Ali, Farooq.,Alam, Sadia.,Shakeela, Qismat.,...&Zaman, Wajid.(2022).Molecular Characterization of Bacterial Isolates from Soil Samples and Evaluation of their Antibacterial Potential against MDRS.MOLECULES,27(19).
MLA	Sadiqi, Shahida,et al."Molecular Characterization of Bacterial Isolates from Soil Samples and Evaluation of their Antibacterial Potential against MDRS".MOLECULES 27.19(2022).