A lineage-specific arginine in POS1 is required for fruit size control in Physaleae (Solanaceae) via gene co-option

doi:10.1111/tpj.15786

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	A lineage-specific arginine in POS1 is required for fruit size control in Physaleae (Solanaceae) via gene co-option
	Wang, Li ; Liu, Xueyang 3; Li, Qiaoru 3; Xu, Nan 3; He, Chaoying 2,3
刊名	PLANT JOURNAL
	2022
卷号	111 期号:1 页码:183-204
关键词	co-option cell expansion functional evolution fruit size POS1 CRF3 Solanaceae
ISSN号	0960-7412
DOI	10.1111/tpj.15786
文献子类	Article
英文摘要	Solanaceae have important economic value mainly due to their edible fruits. Physalis organ size 1/cytokinin response factor 3 (POS1/CRF3), a unique gene in Solanaceae, is involved in fruit size variation in Physalis but not in Solanum. However, the underlying mechanisms remain elusive. Here, we found that POS1/CRF3 was likely created via the fusion of CRF7 and CRF8 duplicates. Multiple genetic manipulations revealed that only POS1 and Capsicum POS1 (CaPOS1) functioned in fruit size control via the positive regulation of cell expansion. Comparative studies in a phylogenetic framework showed the directional enhancement of POS1-like expression in the flowers and fruits of Physaleae and the specific gain of certain interacting proteins associated with cell expansion by POS1 and CaPOS1. A lineage-specific single nucleotide polymorphism (SNP) caused the 68th amino acid histidine in the POS1 orthologs of non-Physaleae (Nicotiana and Solanum) to change to arginine in Physaleae (Physalis and Capsicum). Substituting the arginine in Physaleae POS1-like by histidine completely abolished their function in the fruits and the protein-protein interaction (PPI) with calreticulin-3. Transcriptomic comparison revealed the potential downstream pathways of POS1, including the brassinosteroid biosynthesis pathway. However, POS1-like may have functioned ancestrally in abiotic stress within Solanaceae. Our work demonstrated that heterometric expression and a SNP caused a single amino acid change to establish new PPIs, which contributed to the co-option of POS1 in multiple regulatory pathways to regulate cell expansion and thus fruit size in Physaleae. These results provide new insights into fruit morphological evolution and fruit yield control.
学科主题	Plant Sciences
电子版国际标准刊号	1365-313X
出版地	HOBOKEN
WOS关键词	RECEPTOR KINASE BRI1 ; TOMATO FRUIT ; PROVIDES INSIGHTS ; GENOME SEQUENCE ; MATERNAL CONTROL ; CELL-CYCLE ; SEED SIZE ; EVOLUTION ; EXPRESSION ; CYTOKININ
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	WILEY
WOS记录号	WOS:000793143700001
资助机构	National Natural Science Foundation of China [31930007, 31525003] ; Strategic Priority Research Program of the Chinese Academy of Sciences [XDB27010106]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/28525]
专题	系统与进化植物学国家重点实验室
作者单位	1.Chinese Acad Sci, Innovat Acad Seed Design, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Yuquan Rd 19, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Nanxincun 20, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Wang, Li,Liu, Xueyang,Li, Qiaoru,et al. A lineage-specific arginine in POS1 is required for fruit size control in Physaleae (Solanaceae) via gene co-option[J]. PLANT JOURNAL,2022,111(1):183-204.
APA	Wang, Li,Liu, Xueyang,Li, Qiaoru,Xu, Nan,&He, Chaoying.(2022).A lineage-specific arginine in POS1 is required for fruit size control in Physaleae (Solanaceae) via gene co-option.PLANT JOURNAL,111(1),183-204.
MLA	Wang, Li,et al."A lineage-specific arginine in POS1 is required for fruit size control in Physaleae (Solanaceae) via gene co-option".PLANT JOURNAL 111.1(2022):183-204.