The novel amylase function of the carboxyl terminal domain of Amy63 | |
Sun, Yufan1,2,3; Liu, Ge6,7,8; Liu, Guangfeng4; Tang, Haixu5; Sun, Chaomin6,7,8; Zhang, Wen2,3; Chen, Li1 | |
刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS |
2023-09-03 | |
卷号 | 671页码:10-17 |
关键词 | Amy63 Amylase CTD The smallest amylase Oligomeric assembly Nanozyme X-ray crystallography |
ISSN号 | 0006-291X |
DOI | 10.1016/j.bbrc.2023.05.071 |
通讯作者 | Sun, Chaomin(sunchaomin2020@126.com) ; Zhang, Wen(wenz@fudan.edu.cn) ; Chen, Li(lichen_bk@fudan.edu.cn) |
英文摘要 | a-amylase plays a crucial role in regulating metabolism and health by hydrolyzing of starch and glycogen. Despite comprehensive studies of this classic enzyme spanning over a century, the function of its carboxyl terminal domain (CTD) with a conserved eight b-strands is still not fully understood. Amy63, identified from a marine bacterium, was reported as a novel multifunctional enzyme with amylase, agarase and carrageenase activities. In this study, the crystal structure of Amy63 was determined at 1.8 & ANGS; resolution, revealing high conservation with some other amylases. Interestingly, the independent amylase activity of the carboxyl terminal domain of Amy63 (Amy63_CTD) was newly discovered by the plate-based assay and mass spectrometry. To date, the Amy63_CTD alone could be regarded as the smallest amylase subunit. Moreover, the significant amylase activity of Amy63_CTD was measured over a wide range of temperature and pH, with optimal activity at 60 & DEG;C and pH 7.5. The Small-angle X-ray scattering (SAXS) data showed that the high-order oligomeric assembly gradually formed with increasing concentration of Amy63_CTD, implying the novel catalytic mechanism as revealed by the assembly structure. Therefore, the discovery of the novel independent amylase activity of Amy63_CTD suggests a possible missing step or a new perspective in the complex catalytic process of Amy63 and other related a-amylases. This work may shed light on the design of nanozymes to process marine polysaccharides efficiently. |
资助项目 | National Science and Technology Major Project[2014ZX09101046-004] ; National Natural Science Foundation of China[11179012] ; National Key Basic Research and Development Plan[2011CB710800] ; the Medical Research Data Center in Shanghai Medical College of Fudan University |
WOS关键词 | X-RAY-SCATTERING ; LICHENIFORMIS ALPHA-AMYLASE ; CRYSTAL-STRUCTURE ; DATA REDUCTION ; BINDING ; EVOLUTION |
WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
语种 | 英语 |
出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
WOS记录号 | WOS:001018431500001 |
内容类型 | 期刊论文 |
源URL | [http://ir.qdio.ac.cn/handle/337002/182379] |
专题 | 海洋研究所_实验海洋生物学重点实验室 |
通讯作者 | Sun, Chaomin; Zhang, Wen; Chen, Li |
作者单位 | 1.Fudan Univ, Sch Basic Med Sci, Dept Med Microbiol, Key Lab Med Mol Virol,Minist Educ & Hlth, Shanghai 200032, Peoples R China 2.Fudan Univ, Pudong Med Ctr, Inst Biomed Sci, Shanghai 200032, Peoples R China 3.Fudan Univ, Sch Basic Med Sci, Dept Syst Biol Med, Shanghai 200032, Peoples R China 4.Chinese Acad Sci, Shanghai Adv Res Inst, Natl Ctr Prot Sci Shanghai, Shanghai 201204, Peoples R China 5.Indiana Univ, Luddy Sch Informat Comp & Engn, Bloomington, IN 47408 USA 6.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 7.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 8.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
推荐引用方式 GB/T 7714 | Sun, Yufan,Liu, Ge,Liu, Guangfeng,et al. The novel amylase function of the carboxyl terminal domain of Amy63[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2023,671:10-17. |
APA | Sun, Yufan.,Liu, Ge.,Liu, Guangfeng.,Tang, Haixu.,Sun, Chaomin.,...&Chen, Li.(2023).The novel amylase function of the carboxyl terminal domain of Amy63.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,671,10-17. |
MLA | Sun, Yufan,et al."The novel amylase function of the carboxyl terminal domain of Amy63".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 671(2023):10-17. |
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