Switch of cell migration modes orchestrated by changes of three-dimensional lamellipodium structure and intracellular diffusion

doi:10.1038/s41467-023-40858-x

CORC > 力学研究所 > 中国科学院力学研究所 > 非线性力学国家重点实验室

	Switch of cell migration modes orchestrated by changes of three-dimensional lamellipodium structure and intracellular diffusion
	Jiang, Chao; Luo, Hong-Yu; Xu, Xinpeng; Dou, Shuo-Xing; Li, Wei; Guan DS(关东石); Ye, Fangfu; Chen, Xiaosong; Guo, Ming; Wang, Peng-Ye
刊名	NATURE COMMUNICATIONS
	2023-08-24
卷号	14 期号:1 页码:13
DOI	10.1038/s41467-023-40858-x
通讯作者	Wang, Peng-Ye(pywang@iphy.ac.cn) ; Li, Hui(huili@bnu.edu.cn)
英文摘要	Cell migration plays important roles in many biological processes, but how migrating cells orchestrate intracellular molecules and subcellular structures to regulate their speed and direction is still not clear. Here, by characterizing the intracellular diffusion and the three-dimensional lamellipodium structures of fish keratocyte cells, we observe a strong positive correlation between the intracellular diffusion and cell migration speed and, more importantly, discover a switching of cell migration modes with reversible intracellular diffusion variation and lamellipodium structure deformation. Distinct from the normal fast mode, cells migrating in the newly-found slow mode have a deformed lamellipodium with swollen-up front and thinned-down rear, reduced intracellular diffusion and compartmentalized macromolecule distribution in the lamellipodium. Furthermore, in turning cells, both lamellipodium structure and intracellular diffusion dynamics are also changed, with left-right symmetry breaking. We propose a mechanism involving the front-localized actin polymerization and increased molecular crowding in the lamellipodium to explain how cells spatiotemporally coordinate the intracellular diffusion dynamics and the lamellipodium structure in regulating their migrations.
分类号	一类
资助项目	We thank National Center for Protein Sciences at Peking University, particularly Dr. Siying Qin, for technical help with AFM. We thank Dr. Qihui Fan, Dr. Ruipei Xie, and Yuhang Li for their technical help with micromanipulation. We thank Optofem Technology[12074043] ; We thank National Center for Protein Sciences at Peking University, particularly Dr. Siying Qin, for technical help with AFM. We thank Dr. Qihui Fan, Dr. Ruipei Xie, and Yuhang Li for their technical help with micromanipulation. We thank Optofem Technology[11874415] ; We thank National Center for Protein Sciences at Peking University, particularly Dr. Siying Qin, for technical help with AFM. We thank Dr. Qihui Fan, Dr. Ruipei Xie, and Yuhang Li for their technical help with micromanipulation. We thank Optofem Technology[12135003] ; National Natural Science Foundation of China ; [12122402]
WOS关键词	PARTICLE TRACKING ; ACTIN ; CYTOPLASM ; FLOW ; MECHANICS ; DYNAMICS ; MOTILITY ; DRIVEN ; SHAPE ; TRANSPORT
WOS研究方向	Science & Technology - Other Topics
语种	英语
WOS记录号	WOS:001054501000003
资助机构	We thank National Center for Protein Sciences at Peking University, particularly Dr. Siying Qin, for technical help with AFM. We thank Dr. Qihui Fan, Dr. Ruipei Xie, and Yuhang Li for their technical help with micromanipulation. We thank Optofem Technology ; National Natural Science Foundation of China
其他责任者	Wang, Peng-Ye ; Li, Hui
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/93334]
专题	力学研究所_非线性力学国家重点实验室
推荐引用方式 GB/T 7714	Jiang, Chao,Luo, Hong-Yu,Xu, Xinpeng,et al. Switch of cell migration modes orchestrated by changes of three-dimensional lamellipodium structure and intracellular diffusion[J]. NATURE COMMUNICATIONS,2023,14(1):13.
APA	Jiang, Chao.,Luo, Hong-Yu.,Xu, Xinpeng.,Dou, Shuo-Xing.,Li, Wei.,...&Li, Hui.(2023).Switch of cell migration modes orchestrated by changes of three-dimensional lamellipodium structure and intracellular diffusion.NATURE COMMUNICATIONS,14(1),13.
MLA	Jiang, Chao,et al."Switch of cell migration modes orchestrated by changes of three-dimensional lamellipodium structure and intracellular diffusion".NATURE COMMUNICATIONS 14.1(2023):13.