Expression of secreted antimicrobial peptide in a marine diatom-Phaeodactylum tricornutum | |
Yanga, Wenting1,3,4; Zhou, Lu2,3,4; Zhuo, Jintao2,3,4; Li, Xin2,3,4; Wu, Songcui3,4; Yu, Guihong; Gao, Shan3,4; Wang, Guangce3,4 | |
刊名 | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS |
2023-09-01 | |
卷号 | 75页码:7 |
关键词 | Phaeodactylum tricornutum Secretory antimicrobial peptide Antibacterial |
ISSN号 | 2211-9264 |
DOI | 10.1016/j.algal.2023.103270 |
通讯作者 | Gao, Shan(shangao@qdio.ac.cn) ; Wang, Guangce(gcwang@qdio.ac.cn) |
英文摘要 | Antimicrobial peptides (AMPs) are small-molecule polypeptides that occur widely in nature, have a broad antibacterial spectrum, and do not induce drug resistance in bacteria. Therefore, they are potential substitutes for antibiotics. However, there have been few reports of the production of the AMPs secreted by diatoms. Phaeodactylum tricornutum is a model diatom species for which protocols for its transformation are well established, and it can be directly used as fish and shrimp bait. In this study, we used P. tricornutum as a cell factory to produce secreted AMPs extracellularly. We also constructed an expression vector containing the secretory promoter and signal peptide of the Scygonadin gene, and successfully expressed AMPs in P. tricornutum, confirmed at the levels of DNA, RNA, and protein. Bacteriostatic experiments showed that the secreted AMPs exerted an obvious antibacterial effect on Kocuria sp. and Trelief5 alpha Chemical Competent Cells. This study demonstrates that P. tricornutum effectively expresses and secretes AMPs with obvious antibacterial effects. |
资助项目 | National Natural Science Founda- tion of China[41976097] ; National Key R & D Program of China[2018YFD0901500] |
WOS关键词 | TRANSFORMATION ; MECHANISMS ; GENE |
WOS研究方向 | Biotechnology & Applied Microbiology |
语种 | 英语 |
出版者 | ELSEVIER |
WOS记录号 | WOS:001083333200001 |
内容类型 | 期刊论文 |
源URL | [http://ir.qdio.ac.cn/handle/337002/181622] |
专题 | 海洋研究所_实验海洋生物学重点实验室 |
通讯作者 | Gao, Shan; Wang, Guangce |
作者单位 | 1.Qingdao Agr Univ, Coll Life Sci, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao, Peoples R China |
推荐引用方式 GB/T 7714 | Yanga, Wenting,Zhou, Lu,Zhuo, Jintao,et al. Expression of secreted antimicrobial peptide in a marine diatom-Phaeodactylum tricornutum[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2023,75:7. |
APA | Yanga, Wenting.,Zhou, Lu.,Zhuo, Jintao.,Li, Xin.,Wu, Songcui.,...&Wang, Guangce.(2023).Expression of secreted antimicrobial peptide in a marine diatom-Phaeodactylum tricornutum.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,75,7. |
MLA | Yanga, Wenting,et al."Expression of secreted antimicrobial peptide in a marine diatom-Phaeodactylum tricornutum".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 75(2023):7. |
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