A modified CTAB method for high-molecular-weight DNA preparation from deep-sea holothurians

doi:10.1080/10236244.2023.2241977

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海生物学研究室 > 海洋生物生态与进化研究组

	A modified CTAB method for high-molecular-weight DNA preparation from deep-sea holothurians
	Pu, Yujin 2,3; Liu, Jun 3; Zhang, Nannan 1; Liu, Shanshan 1; Zhang, Haibin 3
刊名	MARINE AND FRESHWATER BEHAVIOUR AND PHYSIOLOGY
	2023-08-03
页码	14
关键词	DNA extraction deep-sea holothurians high-molecular-weight DNA CTAB >
ISSN号	1023-6244
DOI	10.1080/10236244.2023.2241977
通讯作者	Zhang, Haibin(hzhang@idsse.ac.cn)
英文摘要	With the features as global distribution and all depths dwelling, holothurians function an ideal model for the study of deep-sea adaptation. Acquisition of high-molecular-weight DNA is the basic requirement for molecular biology analyses. However, extraction of high-molecular-weight DNA from deep-sea holothurians is difficult by degradation and contamination. Here, we presented a modified CTAB (lysis buffer with cetyl trimethyl ammonium bromide) method and compared its extraction efficiency for deep-sea holothurians with other five common methods, i.e. the Tiangen Biotech kit, Qiagen kit, Omega Bio-tek kit, Sangon Biotech kit, and SDS (lysis buffer with sodium dodecyl sulfate). Based on the results of yield, integrity, and purity, we found that the modified CTAB method is more suitable to extract high-quality DNA for deep-sea holothurians, with a large amount of yield, minimized degradation, and higher purity. This study provides a high-quality DNA of deep-sea holothurians for genome sequencing and further studies.
WOS关键词	ECHINODERMATA HOLOTHUROIDEA ; ELASIPODIDA PSYCHROPOTIDAE ; SPECIES ECHINODERMATA ; MITOCHONDRIAL-DNA ; EXTRACTION ; TISSUES ; STORAGE ; GENUS ; RNA ; DEGRADATION
WOS研究方向	Marine & Freshwater Biology
语种	英语
出版者	TAYLOR & FRANCIS LTD
WOS记录号	WOS:001038633900001
内容类型	期刊论文
源URL	[http://ir.idsse.ac.cn/handle/183446/10420]
专题	深海科学研究部_深海生物学研究室_海洋生物生态与进化研究组
通讯作者	Zhang, Haibin
作者单位	1.BGI, BGI Qingdao, BGI Shenzhen, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya, Peoples R China
推荐引用方式 GB/T 7714	Pu, Yujin,Liu, Jun,Zhang, Nannan,et al. A modified CTAB method for high-molecular-weight DNA preparation from deep-sea holothurians[J]. MARINE AND FRESHWATER BEHAVIOUR AND PHYSIOLOGY,2023:14.
APA	Pu, Yujin,Liu, Jun,Zhang, Nannan,Liu, Shanshan,&Zhang, Haibin.(2023).A modified CTAB method for high-molecular-weight DNA preparation from deep-sea holothurians.MARINE AND FRESHWATER BEHAVIOUR AND PHYSIOLOGY,14.
MLA	Pu, Yujin,et al."A modified CTAB method for high-molecular-weight DNA preparation from deep-sea holothurians".MARINE AND FRESHWATER BEHAVIOUR AND PHYSIOLOGY (2023):14.