Structure and immunoreactivity of purified Siberian apricot (Prunus sibirica L.) kernel allergen under high hydrostatic pressure treatment | |
Zhu, Qianqian2,3; Tang, Xiaohui2; Lu, Mei1; Chen, Jingjing2 | |
刊名 | FOOD BIOSCIENCE |
2022-08-01 | |
卷号 | 48页码:7 |
关键词 | Siberian apricot kernel Purification Identification High hydrostatic pressure Immunoreactivity |
ISSN号 | 2212-4292 |
DOI | 10.1016/j.fbio.2022.101727 |
通讯作者 | Chen, Jingjing |
英文摘要 | In the present study, the allergen in Siberian apricot (Prunus sibirica L.) kernel (SAK) was extracted, purified, and identified. The effect of high-pressure processing on the physiochemical property, structure, as well as the allergenicity of the allergen were determined. The changes in the molecular morphology and secondary structure of Siberian apricot kernels allergen (SAKA) after high hydrostatic pressure (HHP) processing were analyzed using atomic force microscopy and circular dichroism respectively. The immunoreactivity was evaluated by western blotting and indirect-binding enzyme-linked immunosorbent assays. Our result showed that after HHP processing, the intensity of a 40 kDa polypeptide band in SAKA was decreased. The immunoreactivity of SAKA was reduced to 72.74% +/- 1.09% after high pressure processing at 500 MPa for 900 s. The results of this study may provide important implications for identification of apricot kernel allergen as well as the development of apricot kernels foods and beverages with low level of allergenicity. |
资助项目 | National Key R&D Program of China[2017YFC1600902] |
WOS关键词 | SOY PROTEIN ISOLATE ; STRUCTURAL-PROPERTIES ; ALMOND ; AMANDIN ; HEAT |
WOS研究方向 | Food Science & Technology |
语种 | 英语 |
出版者 | ELSEVIER |
WOS记录号 | WOS:000797918200003 |
资助机构 | National Key R&D Program of China |
内容类型 | 期刊论文 |
源URL | [http://ir.idsse.ac.cn/handle/183446/9622] |
专题 | 联合实验室_深海生命研究院 |
通讯作者 | Chen, Jingjing |
作者单位 | 1.Univ Nebraska Lincoln, Dept Food Sci & Technol, Lincoln, NE 68588 USA 2.Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China 3.Chinese Acad Sci, Inst Deep sea Sci & Engn, Sanya 572000, Peoples R China |
推荐引用方式 GB/T 7714 | Zhu, Qianqian,Tang, Xiaohui,Lu, Mei,et al. Structure and immunoreactivity of purified Siberian apricot (Prunus sibirica L.) kernel allergen under high hydrostatic pressure treatment[J]. FOOD BIOSCIENCE,2022,48:7. |
APA | Zhu, Qianqian,Tang, Xiaohui,Lu, Mei,&Chen, Jingjing.(2022).Structure and immunoreactivity of purified Siberian apricot (Prunus sibirica L.) kernel allergen under high hydrostatic pressure treatment.FOOD BIOSCIENCE,48,7. |
MLA | Zhu, Qianqian,et al."Structure and immunoreactivity of purified Siberian apricot (Prunus sibirica L.) kernel allergen under high hydrostatic pressure treatment".FOOD BIOSCIENCE 48(2022):7. |
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