Binding of different hyaluronan to CD44 mediates distinct cell adhesion dynamics under shear flow

doi:10.1111/febs.16882

	Binding of different hyaluronan to CD44 mediates distinct cell adhesion dynamics under shear flow
	Li LD(李林达); Ding QH(丁奇寒); Wu Y(武亿); Zheng Z(郑植); Zhang XN(张晓宁); Zhang MK(张明焜); Long M(龙勉); Lv SQ(吕守芹)
刊名	FEBS JOURNAL
	2023-06-16
关键词	CD44 HA molecular weight shear flow two-dimensional interaction
ISSN号	1742-464X
DOI	10.1111/febs.16882
英文摘要	As a known receptor-ligand pair for mediating cell-cell or cell-extracellular matrix adhesions, cluster of differentiation 44 (CD44)-hyaluronan (HA) interactions are not only determined by molecular weight (MW) diversity of HA, but also are regulated by external physical or mechanical factors. However, the coupling effects of HA MW and shear flow are still unclear. Here, we compared the differences between high molecular weight HA (HHA) and low molecular weight HA (LHA) binding to CD44 under varied shear stresses. The results demonstrated that HHA dominated the binding phase but LHA was in favour of the shear resistance phase, respectively, under shear stress range <= 1.0 dyne center dot cm(-2). This difference was attributed to the high binding strength of the CD44-HHA interaction, as well as the optimal distribution matching between both CD44 and HA sides. Activation of the intracellular signal pathway was sensitive to both HA MW and shear flow. Our findings also indicate that only CD44-HHA interaction under shear stress of 0.2 dyne center dot cm(-2) could significantly enhance the clustering of CD44, as well as induce the increase in both CD44 and CD18 expression. The present study offers the basis for further quantification of the features of CD44-HA interactions and their biological functions.
分类号	二类
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
WOS记录号	WOS:001007367100001
资助机构	National Natural Science Foundation of China [32130061, 11972042, 12172366] ; Scientific Instrument Developing Project of Chinese Academy of Sciences [GJJSTD20220002]
其他责任者	Long, M ; Ln, SQ (corresponding author), Chinese Acad Sci, Inst Mech, Beijing 100190, Peoples R China.
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/92433]
专题	力学研究所_国家微重力实验室
作者单位	1.{Ding Qihan, Wu Yi, Zheng Zhi, Zhang Xiaoning, Zhang Mingkun, Long Mian, Lu Shouqin} Univ Chinese Acad Sci Sch Engn Sci Beijing Peoples R China 2.{Li Linda} Chongqing Univ Coll Bioengn Key Lab Biorheol Sci & Technol Minist Educ Chongqing Peoples R China 3.{Li Linda, Ding Qihan, Wu Yi, Zheng Zhi, Zhang Xiaoning, Zhang Mingkun, Long Mian, Lu Shouqin} Chinese Acad Sci Inst Mech CAS Ctr Excellence Complex Syst Mech Beijing Peoples R China 4.{Li Linda, Ding Qihan, Wu Yi, Zheng Zhi, Zhang Xiaoning, Zhang Mingkun, Long Mian, Lu Shouqin} Chinese Acad Sci Inst Mech Ctr Biomech & BioengnBeijing Key Lab Engn Constr Key Lab MicrogravNatl Micrograv Lab Beijing Peoples R China
推荐引用方式 GB/T 7714	Li LD,Ding QH,Wu Y,et al. Binding of different hyaluronan to CD44 mediates distinct cell adhesion dynamics under shear flow[J]. FEBS JOURNAL,2023.
APA	李林达.,丁奇寒.,武亿.,郑植.,张晓宁.,...&吕守芹.(2023).Binding of different hyaluronan to CD44 mediates distinct cell adhesion dynamics under shear flow.FEBS JOURNAL.
MLA	李林达,et al."Binding of different hyaluronan to CD44 mediates distinct cell adhesion dynamics under shear flow".FEBS JOURNAL (2023).