Biphenyl-degrading Bacteria Isolation with Laser Induced Visualized Ejection Separation Technology and Traditional Colony Sorting

doi:10.1007/s00128-022-03574-8

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	Biphenyl-degrading Bacteria Isolation with Laser Induced Visualized Ejection Separation Technology and Traditional Colony Sorting
	A. L. Li; K. Z. Chen; B. Li; P. Liang and C. F. Shen
刊名	Bulletin of Environmental Contamination and Toxicology
	2022
卷号	109 期号:3 页码:571-576
ISSN号	0007-4861
DOI	10.1007/s00128-022-03574-8
英文摘要	In this work, biphenyl was used as carbon source to enrich microorganisms from polychlorinated biphenyls (PCBs)-contaminated paddy soil samples, and the taxonomic structures in both of the soil samples and the fourth-generation enrichments were examined with high-throughput sequencing. Single cells were isolated from the enrichments via single cell sorting technology named Laser Induced Visualized Ejection Separation Technology (LIVEST) and also traditional single colony sorting, and the genera of the isolates were identified using 16S rRNA sequencing. The results from high-throughput sequencing present that enrichment from generation to generation can considerably change the microbial community. Comparing the two sorting methods, the LIVEST is more time-saving and cell-targeted for microbial resource exploration. Based on the further verification of biphenyl degradation, it was found that some strains belonging to genera Macrococcus, Aerococcus and Metabacillus are capable in degrading biphenyl, which have not been reported yet.
URL标识	查看原文
语种	英语
内容类型	期刊论文
源URL	[http://ir.ciomp.ac.cn/handle/181722/66441]
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	A. L. Li,K. Z. Chen,B. Li,et al. Biphenyl-degrading Bacteria Isolation with Laser Induced Visualized Ejection Separation Technology and Traditional Colony Sorting[J]. Bulletin of Environmental Contamination and Toxicology,2022,109(3):571-576.
APA	A. L. Li,K. Z. Chen,B. Li,&P. Liang and C. F. Shen.(2022).Biphenyl-degrading Bacteria Isolation with Laser Induced Visualized Ejection Separation Technology and Traditional Colony Sorting.Bulletin of Environmental Contamination and Toxicology,109(3),571-576.
MLA	A. L. Li,et al."Biphenyl-degrading Bacteria Isolation with Laser Induced Visualized Ejection Separation Technology and Traditional Colony Sorting".Bulletin of Environmental Contamination and Toxicology 109.3(2022):571-576.