Characterizing the Status of Energetic Metabolism of Dinoflagellate Resting Cysts under Mock Conditions of Marine Sediments via Physiological and Transcriptional Measurements

doi:10.3390/ijms232315033

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	Characterizing the Status of Energetic Metabolism of Dinoflagellate Resting Cysts under Mock Conditions of Marine Sediments via Physiological and Transcriptional Measurements
	Li, Fengting 2,3,4; Yue, Caixia 1,4; Deng, Yunyan 2,3,4; Tang, Ying Zhong 2,3,4
刊名	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
	2022-12-01
卷号	23 期号:23 页码:16
关键词	ATP content energetic metabolism resting cyst Scrippsiella acuminata tricarboxylic acid cycle viability
DOI	10.3390/ijms232315033
通讯作者	Deng, Yunyan(yunyandeng@qdio.ac.cn) ; Tang, Ying Zhong(yingzhong.tang@qdio.ac.cn)
英文摘要	Similar to the seeds of higher plants, resting cysts, a non-motile, benthic, and dormant stage in the life history of many dinoflagellate species, play vital roles via germination in the seasonal dynamics and particularly the initiation of harmful algal blooms (HABs) of dinoflagellates. It is thus crucial for resting cysts to balance between the energetic catabolism for viability maintenance and the energy preservation for germination during their dormancy. Despite this importance, studies on how resting cysts of dinoflagellates accomplish energetic metabolism in marine sediment have been virtually absent. In this study, using the cosmopolitan HABs-causing species Scrippsiella acuminata as a representative, we measured the transcriptional activity of the most efficient pathway of the energy catabolism tricarboxylic acid (TCA) cycle, cell viability (via neutral red staining), and the cellular ATP content of resting cysts under a set of mock conditions in marine sediments (e.g., 4 degrees C, darkness, and anoxia) for a maximum period of one year. Based on the correlation analyses among the expression levels of genes, cyst viability, and ATP content, we revealed that the TCA cycle was still a crucial pathway of energetic catabolism for resting cysts under aerobic conditions, and its expression was elevated at higher temperatures, light irradiation, and the early stage of dormancy. Under anaerobic conditions, however, the TCA cycle pathway ceased expression in resting cysts, as also supported by ATP measurements. Our results have laid a cornerstone for the comprehensive revelation of the energetic metabolism and biochemical processes of dormancy of resting cysts in marine sediments.
资助项目	Key Deployment Project of Centre for Ocean Mega - Research of Science, Chinese Academy of Sciences[COMS2019Q09] ; National Science Foundation of China[42176207] ; Marine Samp;T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2021QNLM040001]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	MDPI
WOS记录号	WOS:000897362700001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/181079]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Deng, Yunyan; Tang, Ying Zhong
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Li, Fengting,Yue, Caixia,Deng, Yunyan,et al. Characterizing the Status of Energetic Metabolism of Dinoflagellate Resting Cysts under Mock Conditions of Marine Sediments via Physiological and Transcriptional Measurements[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2022,23(23):16.
APA	Li, Fengting,Yue, Caixia,Deng, Yunyan,&Tang, Ying Zhong.(2022).Characterizing the Status of Energetic Metabolism of Dinoflagellate Resting Cysts under Mock Conditions of Marine Sediments via Physiological and Transcriptional Measurements.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,23(23),16.
MLA	Li, Fengting,et al."Characterizing the Status of Energetic Metabolism of Dinoflagellate Resting Cysts under Mock Conditions of Marine Sediments via Physiological and Transcriptional Measurements".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23.23(2022):16.