Pooled resequencing of larvae and adults reveals genomic variations associated with Ostreid herpesvirus 1 resistance in the Pacific oyster Crassostrea gigas

doi:10.3389/fimmu.2022.928628

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Pooled resequencing of larvae and adults reveals genomic variations associated with Ostreid herpesvirus 1 resistance in the Pacific oyster Crassostrea gigas
	Yao, Shanshan 5,6,7,8,9; Li, Li 4,6,7,8,9; Guan, Xudong 7,8,9; He, Yan 3; Jouaux, Aude 2; Xu, Fei 7,8,9; Guo, Ximing 1; Zhang, Guofan 4,6,7,8,9; Zhang, Linlin 4,6,7,8,9
刊名	FRONTIERS IN IMMUNOLOGY
	2022-08-19
卷号	13 页码:17
关键词	Ostreid herpesvirus antiviral innate immunity oyster pooled-resequencing transcriptomic response disease resistance larval mortality molluscan aquaculture
ISSN号	1664-3224
DOI	10.3389/fimmu.2022.928628
通讯作者	Guo, Ximing(xguo@hsrl.rutgers.edu) ; Zhang, Guofan(gzhang@qdio.ac.cn) ; Zhang, Linlin(linlinzhang@qdio.ac.cn)
英文摘要	The Ostreid herpesvirus 1 (OsHV-1) is a lethal pathogen of the Pacific oyster (Crassostrea gigas), an important aquaculture species. To understand the genetic architecture of the defense against the pathogen, we studied genomic variations associated with herpesvirus-caused mortalities by pooled whole-genome resequencing of before and after-mortality larval samples as well as dead and surviving adults from a viral challenge. Analysis of the resequencing data identified 5,271 SNPs and 1,883 genomic regions covering 3,111 genes in larvae, and 18,692 SNPs and 28,314 regions covering 4,863 genes in adults that were significantly associated with herpesvirus-caused mortalities. Only 1,653 of the implicated genes were shared by larvae and adults, suggesting that the antiviral response or resistance in larvae and adults involves different sets of genes or differentiated members of expanded gene families. Combined analyses with previous transcriptomic data from challenge experiments revealed that transcription of many mortality-associated genes was also significantly upregulated by herpesvirus infection confirming their importance in antiviral response. Key immune response genes especially those encoding antiviral receptors such as TLRs and RLRs displayed strong association between variation in regulatory region and herpesvirus-caused mortality, suggesting they may confer resistance through transcriptional modulation. These results point to previously undescribed genetic mechanisms for disease resistance at different developmental stages and provide candidate polymorphisms and genes that are valuable for understanding antiviral immune responses and breeding for herpesvirus resistance.
资助项目	Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology(Qingdao)[2022QNLM030004] ; Strategic Priority Research Program of the Chinese Academy of Sciences[41976088] ; National Natural Science Foundation of China[XDB42000000] ; Key Development Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Science[COMS2019R01] ; Fonds Europeen de Developpement Regional ; Conseil Regional de Basse-Normandie ; USDA-NIFA Hatch ; NJAES Animal Health Project[1004475/NJ32920]
WOS研究方向	Immunology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000849187900001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/180897]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Guo, Ximing; Zhang, Guofan; Zhang, Linlin
作者单位	1.Rutgers State Univ, Dept Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA 2.Univ Caen Basse Normandie, CNRS-7208, UMR, MNHN,UPMC,UCBN,BOREA Biol Organismes & Ecosyst Aqu, Esplanade Paix, Caen, France 3.Ocean Univ China, Coll Marine Life Sci, Minist Educ MOE, Key Lab Mol Genet & Breeding, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Coll Marine Sci, Beijing, Peoples R China 5.Qingdao Univ, Coll Life Sci, Qingdao, Peoples R China 6.Lab Marine Biol & Biotechnol, Qingdao Natl Lab Marine Sci & Technol, Qingdao, Peoples R China 7.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Ctr Ocean Mega Sci, Qingdao, Peoples R China 8.Chinese Acad Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 9.Chinese Acad Sci, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Yao, Shanshan,Li, Li,Guan, Xudong,et al. Pooled resequencing of larvae and adults reveals genomic variations associated with Ostreid herpesvirus 1 resistance in the Pacific oyster Crassostrea gigas[J]. FRONTIERS IN IMMUNOLOGY,2022,13:17.
APA	Yao, Shanshan.,Li, Li.,Guan, Xudong.,He, Yan.,Jouaux, Aude.,...&Zhang, Linlin.(2022).Pooled resequencing of larvae and adults reveals genomic variations associated with Ostreid herpesvirus 1 resistance in the Pacific oyster Crassostrea gigas.FRONTIERS IN IMMUNOLOGY,13,17.
MLA	Yao, Shanshan,et al."Pooled resequencing of larvae and adults reveals genomic variations associated with Ostreid herpesvirus 1 resistance in the Pacific oyster Crassostrea gigas".FRONTIERS IN IMMUNOLOGY 13(2022):17.