Cell senescence alters responses of porcine trabecular meshwork cells to shear stress

doi:10.3389/fcell.2022.1083130

CORC > 力学研究所 > 中国科学院力学研究所 > 非线性力学国家重点实验室

	Cell senescence alters responses of porcine trabecular meshwork cells to shear stress
	Du, Ruotian 4; Li, Dongyan 4; Zhu, Meng 4; Zheng, Lisha 4; Ren, Keli 3; Han, Dong 3; Li, Long 1,2; Ji, Jing 4; Fan, Yubo 4
刊名	FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
	2022-11-21
卷号	10 页码:14
关键词	shear stress senescence trabecular meshwork cell glaucoma mechanobiology
ISSN号	2296-634X
DOI	10.3389/fcell.2022.1083130
通讯作者	Li, Long(lilong@lnm.imech.ac.cn) ; Ji, Jing(09714@buaa.edu.cn) ; Fan, Yubo(yubofan@buaa.edu.cn)
英文摘要	Mechanical microenvironment and cellular senescence of trabecular meshwork cells (TMCs) are suspected to play a vital role in primary open-angle glaucoma pathogenesis. However, central questions remain about the effect of shear stress on TMCs and how aging affects this process. We have investigated the effect of shear stress on the biomechanical properties and extracellular matrix regulation of normal and senescent TMCs. We found a more significant promotion of Fctin formation, a more obvious realignment of F-actin fibers, and a more remarkable increase in the stiffness of normal cells in response to the shear stress, in comparison with that of senescent cells. Further, as compared to normal cells, senescent cells show a reduced extracellular matrix turnover after shear stress stimulation, which might be attributed to the different phosphorylation levels of the extracellular signal-regulated kinase. Our results suggest that TMCs are able to sense and respond to the shear stress and cellular senescence undermines the mechanobiological response, which may lead to progressive failure of cellular TM function with age.
资助项目	National Natural Science Foundation of China ; Youth Innovation Promotion Association CAS ; [12232019] ; [U20A20390] ; [11827803] ; [11902327] ; [12272388]
WOS关键词	SIGNAL-REGULATED KINASE ; OPEN-ANGLE GLAUCOMA ; ENDOTHELIAL-CELLS ; OXIDATIVE STRESS ; MATRIX METALLOPROTEINASES ; OUTFLOW FACILITY ; AQUEOUS OUTFLOW ; GENE-EXPRESSION ; LAMINA-CRIBROSA ; STIFFNESS
WOS研究方向	Cell Biology ; Developmental Biology
语种	英语
WOS记录号	WOS:000894046900001
资助机构	National Natural Science Foundation of China ; Youth Innovation Promotion Association CAS
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/91210]
专题	力学研究所_非线性力学国家重点实验室
通讯作者	Li, Long; Ji, Jing; Fan, Yubo
作者单位	1.Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing, Peoples R China 2.Chinese Acad Sci, State Key Lab Nonlinear Mech, Beijing, Peoples R China 3.Natl Ctr Nanosci & Technol, Lab Biol Imaging & Nanomed, Beijing, Peoples R China 4.Beihang Univ, Beijing Adv Innovat Ctr Biomed Engn, Sch Biol Sci & Med Engn, Key Lab Biomech Mechanobiol,Minist Educ, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Du, Ruotian,Li, Dongyan,Zhu, Meng,et al. Cell senescence alters responses of porcine trabecular meshwork cells to shear stress[J]. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY,2022,10:14.
APA	Du, Ruotian.,Li, Dongyan.,Zhu, Meng.,Zheng, Lisha.,Ren, Keli.,...&Fan, Yubo.(2022).Cell senescence alters responses of porcine trabecular meshwork cells to shear stress.FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY,10,14.
MLA	Du, Ruotian,et al."Cell senescence alters responses of porcine trabecular meshwork cells to shear stress".FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY 10(2022):14.