Knockout of a diatom cryptochrome by CRISPR/Cas9 causes an increase in light-harvesting protein levels and accumulation of fucoxanthin

doi:10.1016/j.algal.2022.102822

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Knockout of a diatom cryptochrome by CRISPR/Cas9 causes an increase in light-harvesting protein levels and accumulation of fucoxanthin
	Yang, Wenting 2,3,4; Zhou, Lu 1,2,3; Wang, Jing 3; Wang, Lijun 2,3; Gao, Shan 2,3; Wang, Guangce 2,3
刊名	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
	2022-07-01
卷号	66 页码:9
关键词	Fucoxanthin Cryptochrome Phaeodactylum tricornutum
ISSN号	2211-9264
DOI	10.1016/j.algal.2022.102822
通讯作者	Gao, Shan(shangao@qdio.ac.cn) ; Wang, Guangce(gcwang@qdio.ac.cn)
英文摘要	Diatoms are a promising source of fucoxanthin, a carotenoid that has many beneficial effects in human health. However, the gene encoding the last step of the fucoxanthin biosynthesis pathway in diatoms remains uniden-tified, and the molecular regulatory mechanisms are unknown. In this study, we show that cryptochrome CryP, a blue light-sensitive protein in the diatom Phaeodactylum tricornutum regulates fucoxanthin biosynthesis. CryP transcripts showed a diurnal rhythmic expression pattern; however, CryP protein showed high stability under different photoperiods. Furthermore, CryP not only regulates the expression of genes encoding carotenoid biosynthesis, but also affects the level of fucoxanthin chlorophyll a/c-binding proteins (FCPs). Upon knockout of the CryP gene, no change in cell morphology or growth was observed. However, both fucoxanthin content and FCP levels in P. tricornutum knockout lines increased greatly compared with the wild type. Our study is the first to report cryptochrome knockout in diatoms, which opens the possibility of fucoxanthin production through genome editing in P. tricornutum targeting known crucial steps.
资助项目	National Key Research and Development Program of China[2018YFD0901500] ; National Natural Science Foundation of China[41976097] ; National Natural Science Foundation of China[41876163] ; Major Scientific and Technological Innovation Projects in Shandong Province[2019JZZY010815] ; Research Fund for the Taishan Scholar Project of Shandong Province[tspd20210316] ; Key Deployment Project of the Centre for Ocean Mega-Research of Science ; Chinese Academy of Sciences[COMS2019Q02] ; China Agriculture Research System of MOF and MARA[CARS-50]
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000861083100005
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/180717]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Gao, Shan; Wang, Guangce
作者单位	1.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao, Peoples R China 4.Qingdao Agr Univ, Coll Life Sci, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Yang, Wenting,Zhou, Lu,Wang, Jing,et al. Knockout of a diatom cryptochrome by CRISPR/Cas9 causes an increase in light-harvesting protein levels and accumulation of fucoxanthin[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2022,66:9.
APA	Yang, Wenting,Zhou, Lu,Wang, Jing,Wang, Lijun,Gao, Shan,&Wang, Guangce.(2022).Knockout of a diatom cryptochrome by CRISPR/Cas9 causes an increase in light-harvesting protein levels and accumulation of fucoxanthin.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,66,9.
MLA	Yang, Wenting,et al."Knockout of a diatom cryptochrome by CRISPR/Cas9 causes an increase in light-harvesting protein levels and accumulation of fucoxanthin".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 66(2022):9.