Synthesizing glycine betaine via choline oxidation pathway as an osmoprotectant strategy in Haloferacales

doi:10.1016/j.gene.2022.146886

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	Synthesizing glycine betaine via choline oxidation pathway as an osmoprotectant strategy in Haloferacales
	Yang, Na 1,2,3; Ding, Runting 1,2,4; Liu, Jianguo 1,2,3
刊名	GENE
	2022-12-30
卷号	847 页码:9
关键词	Haloferacales Osmoadaptation Glycine betaine Choline oxidation pathway Phylogenetic distribution
ISSN号	0378-1119
DOI	10.1016/j.gene.2022.146886
通讯作者	Liu, Jianguo(jgliu@qdio.ac.cn)
英文摘要	The accumulation of organic compatible solutes, such as glycine betaine, is one of the osmoprotective strategies used by halophilic archaea to adapt to high salinity. The uptake of glycine betaine from the external environment using various transporters has been widely studied in different halophilic archaea. However, the de novo biosynthesis of glycine betaine and its distribution in halophilic archaea remain unclear. In this study, an extremely halophilic archaea strain, named Halorubrum sp. 2020YC2 and previously isolated from a salt-lake sample, was identified with complete choline oxidation pathway genes. Halorubrum sp. 2020YC2 could syn-thesize and accumulate 1.56-4.25 mu mol per mg of protein of glycine betaine in a defined medium, with its content increasing along with increasing salinity. The intracellular content of glycine betaine remained relatively stable at different salinities when another exogenous solute such as trehalose was provided. The metabolic profile and transcriptional results strongly suggested that the intracellular glycine betaine was derived from serine, which came from the glycolytic intermediate 3-phosphoglycerate when glucose was used as the sole carbon source. Out of 205 available genomes of halophilic archaea, genes encoding the choline oxidation pathway were identified in 30 genomes, and more than half of the strains belonging to order Haloferacales contained the choline oxidation pathway. Phylogenetic analysis further indicated that this pathway evolved from halophilic Proteo-bacteria, and its absence in some genera indicated a possible gene loss event during evolution. The analysis of reported culture data of halophilic archaea strains eventually demonstrated that the presence of the choline oxidation pathway had no significant effects on the adaptation of Haloferacales to high salinity habitats. Therefore, the de novo biosynthesis of glycine betaine via the choline oxidation pathway could be an auxiliary osmoprotective strategy in halophilic archaea.
资助项目	Pilot National Laboratory for Marine Science and Technology (Qingdao), China[YQ2018NO04] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB42000000] ; Young Scientists Fund of LMBB
WOS研究方向	Genetics & Heredity
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000867344400001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/180614]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Liu, Jianguo
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Chinese Acad Sci, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Yang, Na,Ding, Runting,Liu, Jianguo. Synthesizing glycine betaine via choline oxidation pathway as an osmoprotectant strategy in Haloferacales[J]. GENE,2022,847:9.
APA	Yang, Na,Ding, Runting,&Liu, Jianguo.(2022).Synthesizing glycine betaine via choline oxidation pathway as an osmoprotectant strategy in Haloferacales.GENE,847,9.
MLA	Yang, Na,et al."Synthesizing glycine betaine via choline oxidation pathway as an osmoprotectant strategy in Haloferacales".GENE 847(2022):9.