The synthesis, characterization and immunological activity of mucopolysaccharide-quaternized chitosan nanoparticles

doi:10.1016/j.ijbiomac.2022.08.069

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	The synthesis, characterization and immunological activity of mucopolysaccharide-quaternized chitosan nanoparticles
	Xu, Chaojie 1,2,3,4; Xing, Ronge 1,2,3; Liu, Song 1,2,3; Qin, Yukun 1,2,3; Li, Kecheng 1,2,3; Yu, Huahua 1,2,3; Li, Pengcheng 1,2,3
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2022-11-01
卷号	220 页码:258-266
关键词	Mucopolysaccharide Quaternized chitosan Nanoparticles DCS cells Immunocompetence
ISSN号	0141-8130
DOI	10.1016/j.ijbiomac.2022.08.069
通讯作者	Xing, Ronge(xingronge@qdio.ac.cn) ; Li, Pengcheng(pcli@qdio.ac.cn)
英文摘要	In this study, nanoparticles were prepared by using positively charged quaternized chitosan and negatively charged mucopolysaccharide such as chondroitin sulfate, heparin and hyaluronic acid. The nanoparticles have a stable nanostructure with particle size in 336.2-424.5 nm, potential in 18.5-31.1 mV and polydispersity index PDI of 0.172-0.335. Moreover, their encapsulation efficiency was 68.77 % and 64.89 %, and they have low endotoxin and good stability. It can significantly promote the expression of IL-6, TNF-alpha, and IL-1 beta of DCS cells. Moreover, the in vivo immune activity of heparin-quaternized chitosan-OVA nanoparticles against BALB/C mice was showed that, the nanoparticles could significantly promote the secretion of immunoglobulins in mice including IgG1 and IgG2. And nanoparticle also can promote the production of immune factors. Meanwhile, the expression of immune factor genes was also elevated. Furthermore, the results of tissue section experiments showed that the nanoparticles are safety of the body.
资助项目	Key deployment projects of Marine Science Research Center of Chinese Academy of Sciences[COMS2020J04]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000862931600008
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/180569]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Xing, Ronge; Li, Pengcheng
作者单位	1.Chinese Acad Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Xu, Chaojie,Xing, Ronge,Liu, Song,et al. The synthesis, characterization and immunological activity of mucopolysaccharide-quaternized chitosan nanoparticles[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2022,220:258-266.
APA	Xu, Chaojie.,Xing, Ronge.,Liu, Song.,Qin, Yukun.,Li, Kecheng.,...&Li, Pengcheng.(2022).The synthesis, characterization and immunological activity of mucopolysaccharide-quaternized chitosan nanoparticles.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,220,258-266.
MLA	Xu, Chaojie,et al."The synthesis, characterization and immunological activity of mucopolysaccharide-quaternized chitosan nanoparticles".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 220(2022):258-266.