Siglec-15 Silencing Inhibits Cell Proliferation and Promotes Cell Apoptosis by Inhibiting STAT1/STAT3 Signaling in Anaplastic Thyroid Carcinoma | |
Hou, Xiaofeng1,2,3; Chen, Chao2,3; He, Xiaodong1; Lan, Xiabin2,3 | |
刊名 | DISEASE MARKERS |
2022-06-20 | |
卷号 | 2022 |
ISSN号 | 0278-0240 |
DOI | 10.1155/2022/1606404 |
通讯作者 | He, Xiaodong(houxf18@lzu.edu.cn) ; Lan, Xiabin(lanxb99@126.com) |
英文摘要 | Thyroid cancer (THCA) represents a frequently seen endocrine cancer, which can be divided as anaplastic thyroid carcinoma (ATC), follicular thyroid carcinoma (FTC), and papillary thyroid carcinoma (PTC). A total of 362 IDEGs were obtained from TCGA-THCA and IMMPORT databases, which were found to be related to BP, CC, MF, and STAT signaling pathway upon GO functional annotation and KEGG analysis. This work identified 23 survival-related hub genes using WGCNA and uniCOX analysis. In addition, a risk prognosis model was constructed to obtain a signature involving fifteen IDEGs. According to survival and univariate along with multivariate analysis, high-risk patients had markedly dismal prognostic outcome compared with low-risk counterparts. Siglec-15 belongs to one of the fifteen IDEG signature, but the precise biological roles in diverse THCA subtypes are largely unclear. In this work, Siglec-15 expression evidently increased in ATC and FTC samples compared with matched surrounding PTC and THCA samples, which was used as a diagnostic biomarker for THCA. Siglec-15 RNAi significantly inhibited cell proliferation and promoted cell apoptosis. Meanwhile, Siglec-15 knockout suppressed the expression of STAT1, STAT3, and VEGF and promoted that of cleaved caspase-3. In vivo experiments revealed that transfection with vectors expressing STAT1 and STAT3 inhibited the Siglec-15 RNAi-induced inhibition on tumor growth and the increases in CD4(+)/CD8(+) ratio. In conclusion, Siglec-15 expression increases in ATC and FTC, which promotes THCA occurrence via the STAT1/STAT3 signaling, in particular for FTC and ATC. Therefore, it is the possible marker that can be used to diagnose and treat THCA. |
WOS关键词 | CANCER ; IMMUNOTHERAPY |
WOS研究方向 | Biotechnology & Applied Microbiology ; Genetics & Heredity ; Research & Experimental Medicine ; Pathology |
语种 | 英语 |
出版者 | HINDAWI LTD |
WOS记录号 | WOS:000852889200001 |
内容类型 | 期刊论文 |
源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/131682] |
专题 | 中国科学院合肥物质科学研究院 |
通讯作者 | He, Xiaodong; Lan, Xiabin |
作者单位 | 1.Lanzhou Univ, Clin Med Coll 2, Dept Gen Surg, Lanzhou 730030, Gansu, Peoples R China 2.Chinese Acad Sci, Univ Chinese Acad Sci, Zhejiang Canc Hosp,Dept Head & Neck Oncol Surg, Inst Basic Med & Canc IBMC,Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China 3.Key Lab Head & Neck Canc Translat Res Zhejiang Pr, Hangzhou 310022, Zhejiang, Peoples R China |
推荐引用方式 GB/T 7714 | Hou, Xiaofeng,Chen, Chao,He, Xiaodong,et al. Siglec-15 Silencing Inhibits Cell Proliferation and Promotes Cell Apoptosis by Inhibiting STAT1/STAT3 Signaling in Anaplastic Thyroid Carcinoma[J]. DISEASE MARKERS,2022,2022. |
APA | Hou, Xiaofeng,Chen, Chao,He, Xiaodong,&Lan, Xiabin.(2022).Siglec-15 Silencing Inhibits Cell Proliferation and Promotes Cell Apoptosis by Inhibiting STAT1/STAT3 Signaling in Anaplastic Thyroid Carcinoma.DISEASE MARKERS,2022. |
MLA | Hou, Xiaofeng,et al."Siglec-15 Silencing Inhibits Cell Proliferation and Promotes Cell Apoptosis by Inhibiting STAT1/STAT3 Signaling in Anaplastic Thyroid Carcinoma".DISEASE MARKERS 2022(2022). |
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