Development and validation of an autophagy-related long non-coding RNA prognostic signature for cervical squamous cell carcinoma and endocervical adenocarcinoma

doi:10.3389/fonc.2022.1049773

	Development and validation of an autophagy-related long non-coding RNA prognostic signature for cervical squamous cell carcinoma and endocervical adenocarcinoma
	Zhou, Shuguang 1,2; Zhang, Weiyu 1,2; Cao, Wujun 3; Jin, Qinqin 1,2; Jiang, Xiya 1,2; Jiang, Xiaomin 1,2; Yang, Yinting 1,2; Yao, Hui 1,2; Chen, Guo 1,2; Gao, Wei 1,2
刊名	FRONTIERS IN ONCOLOGY
	2022-11-03
卷号	12
关键词	cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC) long noncoding RNA (lncRNA) autophagy-related genes (ARGs) autophagy-related lncRNAs (ARLs) prognostic signature immune infiltration
ISSN号	2234-943X
DOI	10.3389/fonc.2022.1049773
通讯作者	Zhou, Shuguang(zhoushuguang@ahmu.edu.cn) ; Tong, Zhuting(zhuting_tong@ahmu.edu.cn)
英文摘要	BackgroundIn this study, we aimed to investigate the signature of the autophagy-related lncRNAs (ARLs) and perform integrated analysis with immune infiltration in cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC). Methods and resultsThe UCSC Xena and HADb databases provided the corresponding data. The ARLs were selected via constructing a co-expression network of autophagy-related genes (ARGs) and lncRNAs. Univariate Cox regression analysis combined with LASSO regression and multivariate Cox regression analysis were utilized to screen lncRNAs. The ARL risk signature was established by Cox regression and tested if it was an independent element bound up with patient prognosis. We used the xCell algorithm and ssGSEA to clarify the pertinence between immune infiltration and the expression of ARLs. Finally, we predicted the sensitivity of drug treatment as well as the immune response. Results indicated that the three prognostic ARLs (SMURF2P1, MIR9-3HG, and AC005332.4) possessed significant diversity and constituted the ARL signature. Risk score was an individual element (HR = 2.82, 95% CI = 1.87-4.30; p < 0.001). Immune infiltration analysis revealed significant increases in central memory CD8(+) T cells, endothelial cells, CD8(+) naive T cells, and preadipocytes in the high-risk group (p < 0.05). There were 10 therapeutic agents that varied significantly in their estimated half-maximal inhibitory concentrations in the two groups. According to the experimental validation, we found that SMURF2P1 belongs to the co-stimulatory genes and might assume greater importance in the development of cervical adenocarcinoma. MIR9-3HG and AC005332.4 belonged to the tumor-suppressor genes and they may play a more positive role in cervical squamous cell carcinoma. ConclusionsThis research explored and validated a novel signature of the ARLs, which can be applied to forecast the prognosis of patients with CESC and is closely associated with immune infiltration.
资助项目	Applied Medicine Research Project of Hefei Health Commission ; Research Fund Project of Anhui Medical University ; Natural Science Foundation of Higher Education Institutions of Anhui Province ; [HWKJ2019-172-14] ; [2020xkj236] ; [KJ2021A0352]
WOS关键词	CANCER ; LNCRNA
WOS研究方向	Oncology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000888291700001
资助机构	Applied Medicine Research Project of Hefei Health Commission ; Research Fund Project of Anhui Medical University ; Natural Science Foundation of Higher Education Institutions of Anhui Province
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/131679]
专题	中国科学院合肥物质科学研究院
通讯作者	Zhou, Shuguang; Tong, Zhuting
作者单位	1.Anhui Med Univ, Affiliated Matern & Child Healthcare Hosp, Dept Gynecol, Hefei, Peoples R China 2.Anhui Prov Matern & Child Healthcare Hosp, Dept Gynecol, Hefei, Peoples R China 3.Anhui Prov Matern & Child Healthcare Hosp, Dept Clin Lab, Hefei, Peoples R China 4.Chinese Acad Sci, Inst Hlth & Med Technol, Hefei Inst Phys Sci, Anhui Prov Key Lab Med Phys & Technol, Hefei, Peoples R China 5.Anhui Med Univ, Affiliated Hosp 1, Dept Radiat Oncol, Hefei, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Shuguang,Zhang, Weiyu,Cao, Wujun,et al. Development and validation of an autophagy-related long non-coding RNA prognostic signature for cervical squamous cell carcinoma and endocervical adenocarcinoma[J]. FRONTIERS IN ONCOLOGY,2022,12.
APA	Zhou, Shuguang.,Zhang, Weiyu.,Cao, Wujun.,Jin, Qinqin.,Jiang, Xiya.,...&Tong, Zhuting.(2022).Development and validation of an autophagy-related long non-coding RNA prognostic signature for cervical squamous cell carcinoma and endocervical adenocarcinoma.FRONTIERS IN ONCOLOGY,12.
MLA	Zhou, Shuguang,et al."Development and validation of an autophagy-related long non-coding RNA prognostic signature for cervical squamous cell carcinoma and endocervical adenocarcinoma".FRONTIERS IN ONCOLOGY 12(2022).