Baicalin Ameliorates Radiation-Induced Lung Injury by Inhibiting the CysLTs/CysLT1 Signaling Pathway

doi:10.1155/2022/2765354

	Baicalin Ameliorates Radiation-Induced Lung Injury by Inhibiting the CysLTs/CysLT1 Signaling Pathway
	Bao, Wu-An 1; Wang, Yue-Zhen 1; Zhu, Xiang 2; Lin, Juan 2; Fan, Ju-Fen 1; Yang, Yang 2; Zhou, Xia 1,2
刊名	EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
	2022-06-25
卷号	2022
ISSN号	1741-427X
DOI	10.1155/2022/2765354
通讯作者	Zhou, Xia(zhouxia@zjcc.org.cn)
英文摘要	Objective. Radiation-induced lung injury (RILI) is a common complication of radiotherapy for thoracic tumors. This study investigated the alleviating effect of baicalin (BA) on RILI and its possible mechanism. Methods. RILI model was established by chest irradiation (IR) of C57BL/6 mice for 16 weeks. Different concentrations of BA were administered, and dexamethasone (DXM) was used as a positive control. Then, the lung pathological changes were observed by HE and Masson staining. The levels of TGF-beta, TNF-alpha, IL-1 beta, IL-6, CysLT, LTC4, and LTE4 were measured by ELISA. The CysLT1 expression was detected by qPCR, immunohistochemistry, and western blot. Type II AEC cells were pretreated with LTD-4 to establish the RILI cell model and intervened with different concentrations of BA. Then, the collagen I protein level was measured by ELISA. The CysLT1 and alpha-SMA expression were detected by qPCR, immunofluorescence, and western blot. Results. BA could effectively improve lung histopathological changes and pulmonary fibrosis. In vivo, BA could inhibit the levels of TGF-beta, TNF-alpha, IL-1 beta, and IL-6 and reduce the levels of CysLT, LTC4, and LTE4. In vitro, different concentrations of LTD4 could reduce the viability of type II AEC cells, which could be reversed by the administration of different concentrations of BA. In addition, BA could reduce CysLT1 mRNA, as well as CysLT1 and alpha-SMA protein levels in vitro and in vivo. Conclusion. BA attenuated lung inflammation and pulmonary fibrosis by inhibiting the CysLTs/CysLT1 pathway, thereby protecting against RILI.
资助项目	Zhejiang Provincial Medicinal Health Program[2020KY469] ; Zhejiang Provincial Medicinal Health Program[2021KY097] ; Science and Technology Programof Traditional Chinese Medicine in Zhejiang Province[2020ZA021] ; Science and Technology Programof Traditional Chinese Medicine in Zhejiang Province[2021ZA022]
WOS关键词	INDUCED PULMONARY-FIBROSIS ; CYSTEINYL LEUKOTRIENE 1 ; MODEL
WOS研究方向	Integrative & Complementary Medicine
语种	英语
出版者	HINDAWI LTD
WOS记录号	WOS:000886702300066
资助机构	Zhejiang Provincial Medicinal Health Program ; Science and Technology Programof Traditional Chinese Medicine in Zhejiang Province
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/131603]
专题	中国科学院合肥物质科学研究院
通讯作者	Zhou, Xia
作者单位	1.Univ Chinese Acad Sci, Canc Hosp, Zhejiang Canc Hosp, Dept Radiat Therapy, Hangzhou, Zhejiang, Peoples R China 2.Chinese Acad Sci, Inst Canc & Basic Med IBMC, Hangzhou, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Bao, Wu-An,Wang, Yue-Zhen,Zhu, Xiang,et al. Baicalin Ameliorates Radiation-Induced Lung Injury by Inhibiting the CysLTs/CysLT1 Signaling Pathway[J]. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,2022,2022.
APA	Bao, Wu-An.,Wang, Yue-Zhen.,Zhu, Xiang.,Lin, Juan.,Fan, Ju-Fen.,...&Zhou, Xia.(2022).Baicalin Ameliorates Radiation-Induced Lung Injury by Inhibiting the CysLTs/CysLT1 Signaling Pathway.EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,2022.
MLA	Bao, Wu-An,et al."Baicalin Ameliorates Radiation-Induced Lung Injury by Inhibiting the CysLTs/CysLT1 Signaling Pathway".EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE 2022(2022).