Site-Specific F-19 NMR Method for Detecting Arf6 GEF Activity

doi:10.1021/acs.analchem.1c05563

	Site-Specific F-19 NMR Method for Detecting Arf6 GEF Activity
	Wang, Jiarong 1; Wu, Bo 1; Zhang, Youjia 1,3; Ge, Liang 1; Wang, Junfeng 1,2
刊名	ANALYTICAL CHEMISTRY
	2022-06-14
卷号	94
ISSN号	0003-2700
DOI	10.1021/acs.analchem.1c05563
通讯作者	Wu, Bo(wubo@hmfl.ac.cn) ; Wang, Junfeng(junfeng@hmfl.ac.cn)
英文摘要	Guanine nucleotide exchange factors (GEFs) of small GTPase (sGTPase) coordinate signal networks in normal cells and dysfunction in cancer. Therefore, effective monitoring of GEF activity is very important for studying the regulation of sGTPase signal transduction. In this study, we developed a 1D F-19 NMR-based method for rapid detection of the GEF activity of sGTPases. The activity of Arf6GEF in vitro and cell lysate environment can be conveniently detected by tracking the conformational changes of the Arf6 switch region where a tfmF site-specific F-19 labeling at Phe47 was introduced. This strategy could potentially be applied to monitor the conformational change of Arf6 or other sGTPase and detect the activities of sGTPase regulatory proteins in physiology and pathology environments.
资助项目	National Natural Science Foundation of China[21673244] ; Collaborative Innovation Program of Hefei Science Center, CAS[2020HSC-CIP008] ; High Magnetic Field Laboratory of Anhui Province
WOS关键词	GUANINE-NUCLEOTIDE EXCHANGE ; ACTIVATION ; PROTEINS ; PROBE
WOS研究方向	Chemistry
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000811922800001
资助机构	National Natural Science Foundation of China ; Collaborative Innovation Program of Hefei Science Center, CAS ; High Magnetic Field Laboratory of Anhui Province
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/131267]
专题	中国科学院合肥物质科学研究院
通讯作者	Wu, Bo; Wang, Junfeng
作者单位	1.Chinese Acad Sci, Hefei Inst Phys Sci, High Magnet Field Lab, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Anhui, Peoples R China 2.Anhui Univ, Inst Phys Sci & Informat Technol, Hefei 230031, Anhui, Peoples R China 3.Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Wang, Jiarong,Wu, Bo,Zhang, Youjia,et al. Site-Specific F-19 NMR Method for Detecting Arf6 GEF Activity[J]. ANALYTICAL CHEMISTRY,2022,94.
APA	Wang, Jiarong,Wu, Bo,Zhang, Youjia,Ge, Liang,&Wang, Junfeng.(2022).Site-Specific F-19 NMR Method for Detecting Arf6 GEF Activity.ANALYTICAL CHEMISTRY,94.
MLA	Wang, Jiarong,et al."Site-Specific F-19 NMR Method for Detecting Arf6 GEF Activity".ANALYTICAL CHEMISTRY 94(2022).