Transgenerational reproductive toxicity of 2,4,6-trinitrotoluene (TNT) and its metabolite 4-ADNT in Caenorhabditis elegans

doi:10.1016/j.etap.2022.103865

	Transgenerational reproductive toxicity of 2,4,6-trinitrotoluene (TNT) and its metabolite 4-ADNT in Caenorhabditis elegans
	Ni, Shenyao 2; Zhang, Huijun 4,5; Zhao, Yanan 2; Pei, Chengcheng 2; Nie, Yaguang 2; Liu, Xiaodong 2,4,5; Wu, Lijun ; Xu, An 1,2,3
刊名	ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
	2022-05-01
卷号	92
关键词	TNT 4-ADNT C elegans Reproductive toxicity Transgenerational effects
ISSN号	1382-6689
DOI	10.1016/j.etap.2022.103865
通讯作者	Nie, Yaguang(nyg@ahu.edu.cn) ; Xu, An(anxu@ipp.ac.cn)
英文摘要	2,4,6-trinitrotoluene (TNT) as an energetic compound widely used in military applications has aroused great concerns in recent years due to its large-scale contamination in soil and water; however, its toxicity is still largely unknown. In this study, we investigated the reproductive toxicity and the transgenerational effects of TNT on Caenorhabditis elegans (C. elegans). Our data showed that exposure to TNT at concentrations ranging from 10 to 100 ng/mL resulted in decreasing the lifespan, brood size, number of oocytes and eggs in uterus, while increasing the number of germ cell apoptosis in C. elegans. The apoptotic effects of TNT were blocked in mutants of cep-1 (w40), egl-1 (n487), and hus-1 (op241), indicating conserved genotoxic response genes was involved in mediating TNT-induced germ cell apoptosis. Parental exposure to TNT significantly increased the germ cell apoptosis from P0 to F2 generation, but the toxicity faded away in F3 and F4 generations. Furthermore, TNT was rapidly metabolized in P0, and the accumulation of 4-aminodinitrotoluene (4-ADNT), the main metabolite of TNT in C. elegans, showed a significant decrease from P0 to F1 and a slow decrease in the subsequent generations. Our results demonstrated that ingested TNT can cause severe transgenerational reproductive toxicity and be rapidly converted to 4-ADNT in the nematodes. These data provided basis for future studies on the effects of energetic compounds across generations.
资助项目	Anhui Provincial Key Research and Development Programs[202004i07020015] ; Anhui Provincial Key Research and Development Programs[202004i07020016] ; Key Research Program of the Chinese Academy of Science[KGFZD-135-19-09] ; State Key Laboratory of NBC Protection for Civilian[SKLNBC2019-15] ; National Key Research and Development Program of China[2020YFC1808204]
WOS关键词	DNA-DAMAGE ; LIFE-SPAN ; ENERGETIC COMPOUNDS ; CHRONIC EXPOSURE ; STRESS-RESPONSE ; MODEL ORGANISM ; CELL APOPTOSIS ; SOIL TOXICITY ; EARTHWORM ; TRINITROTOLUENE
WOS研究方向	Environmental Sciences & Ecology ; Pharmacology & Pharmacy ; Toxicology
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000793628700004
资助机构	Anhui Provincial Key Research and Development Programs ; Key Research Program of the Chinese Academy of Science ; State Key Laboratory of NBC Protection for Civilian ; National Key Research and Development Program of China
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/130781]
专题	中国科学院合肥物质科学研究院
通讯作者	Nie, Yaguang; Xu, An
作者单位	1.Chinese Acad Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Anhui, Peoples R China 2.Anhui Univ, Informat Mat & Intelligent Sensing Lab Anhui Prov, Inst Phys Sci & Informat Technol, Hefei 230601, Peoples R China 3.Chinese Acad Sci, Anhui Prov Key Lab Environm Toxicol & Pollut Cont, High Field Magnet Lab, Hefei Inst Phys Sci, Hefei 230031, Anhui, Peoples R China 4.Univ Sci & Technol China, Inst Polar Environm, Sch Earth & Space Sci, Hefei 230026, Anhui, Peoples R China 5.Univ Sci & Technol China, Anhui Key Lab Polar Environm & Global Change, Sch Earth & Space Sci, Hefei 230026, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Ni, Shenyao,Zhang, Huijun,Zhao, Yanan,et al. Transgenerational reproductive toxicity of 2,4,6-trinitrotoluene (TNT) and its metabolite 4-ADNT in Caenorhabditis elegans[J]. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,2022,92.
APA	Ni, Shenyao.,Zhang, Huijun.,Zhao, Yanan.,Pei, Chengcheng.,Nie, Yaguang.,...&Xu, An.(2022).Transgenerational reproductive toxicity of 2,4,6-trinitrotoluene (TNT) and its metabolite 4-ADNT in Caenorhabditis elegans.ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,92.
MLA	Ni, Shenyao,et al."Transgenerational reproductive toxicity of 2,4,6-trinitrotoluene (TNT) and its metabolite 4-ADNT in Caenorhabditis elegans".ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY 92(2022).