A spiropyran with low pK(a) for tracking DNA G-quadruplexes and revealing the dissipation of Delta Psi m with senescence using an in-situ switching strategy

doi:10.1016/j.snb.2022.131618

	A spiropyran with low pK(a) for tracking DNA G-quadruplexes and revealing the dissipation of Delta Psi m with senescence using an in-situ switching strategy
	Tian, Xinrong 4; Li, Jin 1,4; Zhang, Yanhui 4; Gao, Ying 4; Afzal, Muhammad Wasim 4; Wang, Aoli 2; James, Tony D.3; Bai, Yinjuan 4; Guo, Yuan 4
刊名	SENSORS AND ACTUATORS B-CHEMICAL
	2022-05-15
卷号	359
关键词	Fluorescent probes G-quadruplexes Membrane potential of mitochondria Senescence Senescent cells
DOI	10.1016/j.snb.2022.131618
通讯作者	Bai, Yinjuan(baiyinjuan@nwu.edu.cn) ; Guo, Yuan(guoyuan@nwu.edu.cn)
英文摘要	The in-situ fluorescence triggering of bioprobes only using endogenous bioforces is an ideal non-destructive real-time detection method, which is of particular interest to improve the accuracy of clinical diagnosis and treatment. We have recently reported a strategy of spiropyran in-situ switching triggered by endogenous biological forces in vivo to develop optical probes for this purpose. However, such probes, as with all spiropyrans, are sensitive to lysosomal acidity. We here present a spiropyran-based fluorescent probe TANG with low pK(a), which can recognize intranuclear DNA G4s in situ without the aid of exogenous light or chemicals and is as stable to lysosomal acidity due to a decreased pK(a) value (4.3). Interestingly, despite the stability to lysosomal pH environment, the TANG spiropyran can be opened in situ by the negative membrane potential of extranuclear mitochondria (Delta Psi m), causing a ratiometric change in fluorescence signals and providing the in-situ and real-time tracking of Delta Psi m. Of note, ratiometric imaging using TANG indicates that Delta Psi m decreases gradually with cellular senescence, which is to the best of our knowledge the first visualization of such mitochondrion-related aging processes using a ratiometric imaging approach.
资助项目	NSF of China[21977082] ; NSF of China[22037002] ; Natural Science Basic Research Plan in Shaanxi Province of China[2020JC-38] ; Technology Plan Project of Xi'an of China[2019218214GXRC018CG019-GXYD18.1] ; Royal Society
WOS关键词	FLUORESCENT-PROBE ; MITOCHONDRIAL ; PH ; SENSOR ; CELLS
WOS研究方向	Chemistry ; Electrochemistry ; Instruments & Instrumentation
语种	英语
出版者	ELSEVIER SCIENCE SA
WOS记录号	WOS:000860649000007
资助机构	NSF of China ; Natural Science Basic Research Plan in Shaanxi Province of China ; Technology Plan Project of Xi'an of China ; Royal Society
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/129177]
专题	中国科学院合肥物质科学研究院
通讯作者	Bai, Yinjuan; Guo, Yuan
作者单位	1.Xian Univ Sci & Technol, Coll Chem & Chem Engn, Xian 710054, Peoples R China 2.Chinese Acad Sci, Hefei Inst Phys Sci, High Magnet Field Lab, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Peoples R China 3.Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England 4.Northwest Univ, Coll Chem & Mat Sci, Natl Demonstrat Ctr Expt Chem Educ, Key Lab Synthet & Nat Funct Mol,Minist Educ, Xian 710127, Peoples R China
推荐引用方式 GB/T 7714	Tian, Xinrong,Li, Jin,Zhang, Yanhui,et al. A spiropyran with low pK(a) for tracking DNA G-quadruplexes and revealing the dissipation of Delta Psi m with senescence using an in-situ switching strategy[J]. SENSORS AND ACTUATORS B-CHEMICAL,2022,359.
APA	Tian, Xinrong.,Li, Jin.,Zhang, Yanhui.,Gao, Ying.,Afzal, Muhammad Wasim.,...&Guo, Yuan.(2022).A spiropyran with low pK(a) for tracking DNA G-quadruplexes and revealing the dissipation of Delta Psi m with senescence using an in-situ switching strategy.SENSORS AND ACTUATORS B-CHEMICAL,359.
MLA	Tian, Xinrong,et al."A spiropyran with low pK(a) for tracking DNA G-quadruplexes and revealing the dissipation of Delta Psi m with senescence using an in-situ switching strategy".SENSORS AND ACTUATORS B-CHEMICAL 359(2022).