Detection and analysis of phage M13KO7 using biosensor based on imaging ellipsometry

doi:10.1016/j.tsf.2022.139563

	Detection and analysis of phage M13KO7 using biosensor based on imaging ellipsometry
	Qi, Cai 4; Zhang, Yanxu 4; Lei, Wenjing 4; Jin, Gang 1,2,3
刊名	THIN SOLID FILMS
	2022-12-01
卷号	763 页码:8
关键词	Optical biosensor Imaging ellipsometry Microfluidic system Phage M13KO7 Detection
ISSN号	0040-6090
DOI	10.1016/j.tsf.2022.139563
通讯作者	Qi, Cai(qicaisucceed@henu.edu.cn)
英文摘要	Antibody GP8 (anti-GP8) was used as a ligand to detect phage M13KO7 using a biosensor based on imaging ellipsometry (IE) without oriented ligand immobilization. When the phage M13KO7 in a sample interacted with the antibody in a microarray, a complex formed upon their affinity, and the layer covering the surface cell of the interaction became thicker. The variation in layer thickness was represented by gray-scale (or brightness) variation of the IE image. Direct and oriented immobilization of the antibody were analyzed to identify the best binding locations to capture the phage. In direct immobilization, the phage could be captured by anti-GP8 and not antibody GP3 (anti-GP3), which was attributed to different copy numbers of the coat proteins on the body of the phage specific to the two antibodies. This indicated that different binding locations could produce different detection efficiency. In oriented immobilization, the phage could be captured by the protein G-Fc-anti-GP3 model, with a low detection efficiency. In contrast, the avidin-biotin-based oriented immobilization could capture the phage with a high detection efficiency. Specifically, it could expose more Fab domains of anti-GP3 labeled with biotin; one avidin had four biotin-binding sites. To confirm detection and analyze binding location, the phage M13KO7 was imaged by transmission electron microscopy and atomic force microscopy. Our results suggest that binding location plays a significant role in the detection of big targets and contributes to enhancing biosensor detection sensitivity.
资助项目	Fund for Yellow River scholars of Henan University ; Guiyang Science and Technology Planning Project[[2021] S105] ; Guiyang Talent Innovation and Entrepreneur Fundation of China ; Guizhou Province ; [[2020] 4Y188]
WOS关键词	PROTEIN-A ; FORCE ; ANTIBODY ; SURFACE ; IMMOBILIZATION ; VISUALIZATION ; LIGAND
WOS研究方向	Materials Science ; Physics
语种	英语
WOS记录号	WOS:000886685800002
资助机构	Fund for Yellow River scholars of Henan University ; Guiyang Science and Technology Planning Project ; Guiyang Talent Innovation and Entrepreneur Fundation of China ; Guizhou Province
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/91053]
专题	力学研究所_国家微重力实验室
通讯作者	Qi, Cai
作者单位	1.Univ Chinese Acad Sci, Sch Engn Sci, 19 Yu-Quan Rd, Beijing 100049, Peoples R China 2.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Fundamental Phys & Math Sci, 1 Sub Lane Xiangshan, Hangzhou 310024, Zhejiang, Peoples R China 3.Chinese Acad Sci, Inst Mech, NML, 15 Beisihuan West Rd, Beijing 100190, Peoples R China 4.Henan Univ, Henan Macquarie Univ Joint Ctr Biomed Innovat, Sch Life Sci, Jinming Ave, Kaifeng 475004, Henan, Peoples R China
推荐引用方式 GB/T 7714	Qi, Cai,Zhang, Yanxu,Lei, Wenjing,et al. Detection and analysis of phage M13KO7 using biosensor based on imaging ellipsometry[J]. THIN SOLID FILMS,2022,763:8.
APA	Qi, Cai,Zhang, Yanxu,Lei, Wenjing,&Jin, Gang.(2022).Detection and analysis of phage M13KO7 using biosensor based on imaging ellipsometry.THIN SOLID FILMS,763,8.
MLA	Qi, Cai,et al."Detection and analysis of phage M13KO7 using biosensor based on imaging ellipsometry".THIN SOLID FILMS 763(2022):8.