Optimizing cryopreservation of sea cucumber (Apostichopus japonicus) sperm using a programmable freezer and computer-assisted sperm analysis

doi:10.3389/fmars.2022.917045

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	Optimizing cryopreservation of sea cucumber (Apostichopus japonicus) sperm using a programmable freezer and computer-assisted sperm analysis
	Xu, Shuai 2,3,4,5,6,7; Liu, Shilin 2,3,4,5,6,7; Sun, Jingchun 2,4,5,6,7; Zhang, Libin 2,3,4,5,6,7; Lin, Chenggang 2,3,4,5,6,7; Sun, Lina 2,3,4,5,6,7; Xing, Lili 2,3,4,5,6,7; Jiang, Chunxi 2,3,4,5,6,7; Yang, Hongsheng 1,2,3,4,5,6,7
刊名	FRONTIERS IN MARINE SCIENCE
	2022-08-01
卷号	9 页码:17
关键词	Apostichopus japonicus sperm cryopreservation motility CASA programmable freezer
DOI	10.3389/fmars.2022.917045
通讯作者	Yang, Hongsheng()
英文摘要	The sea cucumber Apostichopus japonicus has high nutritional, medicinal, and economic value. However, factors such as overexploitation, climate change, and environmental pollution have resulted in serious germplasm degradation in both farmed and wild A. japonicus, and it has been listed as endangered on the IUCN (International Union for Conservation of Nature) Red List of Threatened Species. Cryopreservation is an important method to protect germplasm resources and solve the problem of germplasm degradation. Using a programmable freezer and computer-aided sperm analysis, we comprehensively studied and screened the factors that affect the post-thaw motility of A. japonicus sperm during cryopreservation. Based on our results, we propose the following optimal cryopreservation procedure for A. japonicus sperm: cryo-diluent composition of 12.5% dimethyl sulfoxide and 0.1 mol/L glucose, with filter-sterilized (the filter mesh size: 0.45 mu m) natural seawater (NSW) as the extender; 1:5 mixing ratio of sperm and cryo-diluent; cooling rate and thawing temperatures of 10 degrees C/min and 20 degrees C, respectively. The post-thaw motility of sperm treated using the optimal procedure was > 65%, the fertilization rate (in the blastocyst stage) was nearly 80%, and the hatching rate (in the early auricularia larva stage) was > 65%. Additionally, frozen sperm that had been cryopreserved for 1 year retained a considerable post-thaw motility and fertilization rate compared to recently cryopreserved sperm. We detected obvious differences in sperm freezability among individual A. japonicus, and cryopreservation caused some damage to the sperm structure. In conclusion, our optimized procedure make large-scale cryopreservation of A. japonicus sperm possible, and our results provide valuable information that could be applied to research and conservation of A. japonicus.
资助项目	Agricultural Seed Project of Shandong Province[2020LZGC015] ; National Key Research and Development Project[2018YFD0901602] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0502] ; Chinese Academy of Sciences Modern Marine Ranching Construction Principles and Engineering Technology Innovation Cross Team Project[Y82327101L] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA24030304] ; International Partners Program of Chinese Academy of Sciences[133137KYSB20180069]
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000844632500001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/179901]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Yang, Hongsheng
作者单位	1.Chinese Acad Sci, Innovat Seed Design, Wuhan, Peoples R China 2.Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Engn Lab Marine Ranching, Qingdao, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 6.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 7.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Xu, Shuai,Liu, Shilin,Sun, Jingchun,et al. Optimizing cryopreservation of sea cucumber (Apostichopus japonicus) sperm using a programmable freezer and computer-assisted sperm analysis[J]. FRONTIERS IN MARINE SCIENCE,2022,9:17.
APA	Xu, Shuai.,Liu, Shilin.,Sun, Jingchun.,Zhang, Libin.,Lin, Chenggang.,...&Yang, Hongsheng.(2022).Optimizing cryopreservation of sea cucumber (Apostichopus japonicus) sperm using a programmable freezer and computer-assisted sperm analysis.FRONTIERS IN MARINE SCIENCE,9,17.
MLA	Xu, Shuai,et al."Optimizing cryopreservation of sea cucumber (Apostichopus japonicus) sperm using a programmable freezer and computer-assisted sperm analysis".FRONTIERS IN MARINE SCIENCE 9(2022):17.