Anti-Inflammatory Activity of Three Triterpene from Hippophae rhamnoides L. in Lipopolysaccharide-Stimulated RAW264.7 Cells

	Anti-Inflammatory Activity of Three Triterpene from Hippophae rhamnoides L. in Lipopolysaccharide-Stimulated RAW264.7 Cells
	Han, Y; Yuan, C; Zhou, XW; Han, YJ; He, YH; Ouyang, J; Zhou, WN; Wang, ZH; Wang, HL; Li, G
刊名	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
	2021
卷号	22 期号:21
英文摘要	Oleanolic acid (OA), asiatic acid (AA), and maslinic acid (MA) are ubiquitous isomeric triterpene phytochemicals with many pharmacological effects. To improve their application value, we used lipopolysaccharide (LPS) to induce RAW264.7 cells and studied the differences in the anti-inflammatory effects of the triterpenes according to their structural differences. MTT, Griess, and immunofluorescence assays, ELISA, flow cytometry, and Western blotting, were performed. The release of LPS-induced pro-inflammatory mediators, such as nitric oxide (NO), inducible nitric oxide synthase (iNOS), and interleukin (IL-6), was significantly inhibited by OA, AA, and MA at the same concentration, and AA and MA promoted the production of anti-inflammatory factor IL-10. OA, AA, and MA inhibited LPS-induced NF-kappa B nuclear translocation in RAW264.7 cells. OA and AA inhibited the phosphorylation of ERK1/2, P38, and JNK1/2 in LPS-stimulated RAW264.7 cells. Moreover, OA increased LPS-induced Nrf2 expression and decreased Keap1 expression in RAW264.7 cells. OA, AA, and MA inhibited LPS-stimulated intracellular reactive oxygen species (ROS) production and alleviated mitochondrial membrane potential depletion. Overall, our data suggested that OA, AA, and MA exhibited significant anti-inflammatory effects in vitro. In particular, OA and AA take effects through the MAPKs, NF-kappa B, and Nrf2 signaling pathways.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/61354]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Han, Y,Yuan, C,Zhou, XW,et al. Anti-Inflammatory Activity of Three Triterpene from Hippophae rhamnoides L. in Lipopolysaccharide-Stimulated RAW264.7 Cells[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2021,22(21).
APA	Han, Y.,Yuan, C.,Zhou, XW.,Han, YJ.,He, YH.,...&Li, G.(2021).Anti-Inflammatory Activity of Three Triterpene from Hippophae rhamnoides L. in Lipopolysaccharide-Stimulated RAW264.7 Cells.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,22(21).
MLA	Han, Y,et al."Anti-Inflammatory Activity of Three Triterpene from Hippophae rhamnoides L. in Lipopolysaccharide-Stimulated RAW264.7 Cells".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22.21(2021).