Combination of Medium- and High-Pressure Liquid Chromatography for Isolation of L-tryptophan (Q-marker) from Medicago sativa Extract

	Combination of Medium- and High-Pressure Liquid Chromatography for Isolation of L-tryptophan (Q-marker) from Medicago sativa Extract
	Zhang, SX; Ma, YG; Ma, RH; Wang, QL; Dang, J
刊名	SEPARATIONS
	2022
卷号	9 期号:9
英文摘要	Medicago sativa (alfalfa) is a widely used animal feed. However, its quality has been difficult to control due to the lack of appropriate marker compounds. Therefore, it is very necessary to select an appropriate quality marker (Q-marker) to control its quality. In this study, medium-pressure liquid chromatography and high-pressure liquid chromatography were employed to effectively prepare the separation of the Q-marker (L-tryptophan) from Medicago sativa. Firstly, using MCI GEL (R) CHP20P as the stationary phase, 2.5 g of the target fraction Fr3 was enriched from crude Medicago sativa extract (2.9 kg) by medium-pressure liquid chromatography. Secondly, Sephadex LH-20 was used to further separate Fr3 fractions, and the Fr34 fraction (358.3 mg) was enriched after 14 repetitions. Lastly, using the ReproSil-Pur C18 AQ preparative column, 63.4 mg of L-tryptophan was obtained by high-pressure liquid chromatography, and the purity was above 95%. The results showed that medium-pressure liquid chromatography (MCI GEL (R) CHP20P and Sephadex LH-20) combined with high-pressure liquid chromatography (ReproSil-Pur C18 AQ) could be used to effectively prepare the Q-marker from natural products with satisfactory purity.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/61127]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhang, SX,Ma, YG,Ma, RH,et al. Combination of Medium- and High-Pressure Liquid Chromatography for Isolation of L-tryptophan (Q-marker) from Medicago sativa Extract[J]. SEPARATIONS,2022,9(9).
APA	Zhang, SX,Ma, YG,Ma, RH,Wang, QL,&Dang, J.(2022).Combination of Medium- and High-Pressure Liquid Chromatography for Isolation of L-tryptophan (Q-marker) from Medicago sativa Extract.SEPARATIONS,9(9).
MLA	Zhang, SX,et al."Combination of Medium- and High-Pressure Liquid Chromatography for Isolation of L-tryptophan (Q-marker) from Medicago sativa Extract".SEPARATIONS 9.9(2022).