Comparison of Random and Site-Directed Immobilization of Antibody for alpha-Fetoprotein Direct Immunoassay by Protein Chip

	Comparison of Random and Site-Directed Immobilization of Antibody for alpha-Fetoprotein Direct Immunoassay by Protein Chip
	Huang,Chenghong ; Tang,Hong ; Chen,Hongjie ; Huang,Xiaorong ; Fang,Ying ; Wang,KangRui ; Men,Rongrong ; Gao,Jie ; Wang,Yang ; Chen YY(陈艳艳)
	2022
会议日期	OCT 22-25, 2021
会议地点	Xian, PEOPLES R CHINA
关键词	protein chip alpha-fetoprotein site-directed immobilization of antibody
页码	148-155
英文摘要	For immunoassay, immobilization of antibody is the most important technique in bioengineering for maintaining its intrinsic activity of antigen binding. The sugar chain in the Fc region carried by antibody can be oxidized into aldehyde group by sodium m-periodate, which can couple with the amino-group on chemistry modified substrate surface through covalent linking. Thereby, in situ site-directed immobilization of the oxidized antibody for only one-step reaction can be performed. Here we propose a facile method that oxidized anti-human alpha-fetoprotein antibody (anti-AFP) was in situ site-directed immobilized on APTES treated silicon substrate utilizing microfluidic channel system for AFP immunoassay. The shape of the immobilized anti-AFP was observed by AFM and imaged by imaging ellipsometry of self-developed protein system. Results shown that the amount of antibody immobilization amount and capacity of antigen binding of random immobilization and site-directed immobilization were separately enhanced 16.0% and 7.0%. the suggested strategy has the advantages of simple, environment-friend and convenient-operation.
会议录	INTEGRATED FERROELECTRICS
资助机构	NSF of Chongqing [cstc2019jscx-msxm0366]; Open Fund of Chongqing Key Laboratory of Industrial Fermentation Microorganism [GYFJWSW-201905]; Project of Scientific and Technological Research Program of Chongqing Municipal Education Commission [KJZD-K201806401, KJZD-K201906401]; graduate Innovation Fund of CQUST [YKJCX2020509, YKJCX2120502, YKJCX2120541, 202111551006]
语种	英语
URL标识	查看原文
WOS记录号	WOS:000805668600015
内容类型	会议论文
源URL	[http://dspace.imech.ac.cn/handle/311007/90524]
专题	力学研究所_国家微重力实验室
作者单位	1.CQUST, Chongqing Key Lab Ind Fermentat Microorganism, Chongqing, Peoples R China 2.Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou, Peoples R China 3.Chinese Acad Sci, Inst Mech, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Huang,Chenghong,Tang,Hong,Chen,Hongjie,et al. Comparison of Random and Site-Directed Immobilization of Antibody for alpha-Fetoprotein Direct Immunoassay by Protein Chip[C]. 见:. Xian, PEOPLES R CHINA. OCT 22-25, 2021.