Extraction, purification, and bioactivities analyses of polysaccharides from Glycyrrhiza uralensis | |
Wang, Yonggang3; Li, Yuanli3; Ma, Xueqing1; Ren, Haiwei3; Fan, Wenguang3; Leng, Feifan3; Yang, Mingjun3; Wang, Xiaoli2 | |
刊名 | INDUSTRIAL CROPS AND PRODUCTS |
2018-10-15 | |
卷号 | 122页码:596-608 |
关键词 | Polysaccharides Optimization Characterization Antioxidant activity |
ISSN号 | 0926-6690 |
DOI | 10.1016/j.indcrop.2018.06.011 |
英文摘要 | Glycyrrhiza uralensis is an important traditional Chinese herbal medicine. Polysaccharide is one of active components of G. uralensis. In this paper, the extraction conditions of crude polysaccharides from G. uralensis (GPsO) were optimized by central composite design (CCD), and Three purified polysaccharide fractions (GPs1, GPs2 and GPs3) were studied based on physicochemical property, monosaccharide composition, molecular weight, structures characteristics and antioxidant activities. Results showed that the yield of GPsO reached at the maximum of 4.23%, when the optimum extraction process was 1:13 g/mL (solid-liquid ratio), 600 W (ultrasonic power) at 70 degrees C for 85 min. Except for GPs2, the others (GPsO, GPs1 and GPs3) are non-reducing sugars. They don't contain starch. GPsO and GPs1 contain protein. The molecular weight, monosaccharide composition and Fourier transform infrared spectroscopy (FT-IR) assay showed that GPs1, GPs2 and GPs3 are pyran polysaccharides with a weight average molecular weight (Mw) of 4.513 x 10(3), 1.378 x 10(5) and 2.084 x 10(5) g/mol, respectively. GPs1, GPs2 and GPs3 mainly contain glucose and galactose, respectively. Scanning electron microscope (SEM) demonstrated that GPs1, GPs2 and GPs3 are flaky with a pore structure. Atomic force microscopy (AFM) displayed GPs1 and GPs2 have a large number of dispersions and a small amount of smaller globular aggregates, while GPs3 is the opposite of the abovementioned result. Besides, the antioxidant activity assay of these polysaccharides illustrated that GPs1 has a strong scavenging effect on DPPH radicals and superoxide radical. |
资助项目 | National Natural Science Foundation of China[31460032] ; National Natural Science Foundation of China[81660581] |
WOS研究方向 | Agriculture |
语种 | 英语 |
出版者 | ELSEVIER SCIENCE BV |
WOS记录号 | WOS:000442067300071 |
状态 | 已发表 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.223/handle/2XXMBERH/32396] |
专题 | 生命科学与工程学院 |
通讯作者 | Wang, Yonggang; Leng, Feifan |
作者单位 | 1.Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Natl Foot & Mouth Dis Reference Lab, 1 Xujiaping, Lanzhou 730046, Gansu, Peoples R China 2.Lanzhou Inst Husb & Pharmaceut Sci CAAS, Lanzhou 730050, Gansu, Peoples R China 3.Lanzhou Univ Technol, Sch Life Sci & Engn, Langongping Rd 287, Lanzhou 730050, Gansu, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Yonggang,Li, Yuanli,Ma, Xueqing,et al. Extraction, purification, and bioactivities analyses of polysaccharides from Glycyrrhiza uralensis[J]. INDUSTRIAL CROPS AND PRODUCTS,2018,122:596-608. |
APA | Wang, Yonggang.,Li, Yuanli.,Ma, Xueqing.,Ren, Haiwei.,Fan, Wenguang.,...&Wang, Xiaoli.(2018).Extraction, purification, and bioactivities analyses of polysaccharides from Glycyrrhiza uralensis.INDUSTRIAL CROPS AND PRODUCTS,122,596-608. |
MLA | Wang, Yonggang,et al."Extraction, purification, and bioactivities analyses of polysaccharides from Glycyrrhiza uralensis".INDUSTRIAL CROPS AND PRODUCTS 122(2018):596-608. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论