Pulsed electromagnetic fields promote bone formation by activating the sAC-cAMP-PKA-CREB signaling pathway

doi:10.1002/jcp.27098

	Pulsed electromagnetic fields promote bone formation by activating the sAC-cAMP-PKA-CREB signaling pathway
	Wang, Yuan-Yuan 2,3; Pu, Xiu-Ying 2; Shi, Wen-Gui 3; Fang, Qing-Qing 3; Chen, Xin-Ru 1; Xi, Hui-Rong 3; Gao, Yu-Hai 3; Zhou, Jian 3; Xian, Cory J.4; Chen, Ke-Ming 3
刊名	JOURNAL OF CELLULAR PHYSIOLOGY
	2019-03
卷号	234 期号:3 页码:2807-2821
关键词	bone formation cAMP osteoblast PEMFs soluble AC
ISSN号	0021-9541
DOI	10.1002/jcp.27098
英文摘要	The application of pulsed electromagnetic fields (PEMFs) in the prevention and treatment of osteoporosis has long been an area of interest. However, the clinical application of PEMFs remains limited because of the poor understanding of the PEMF action mechanism. Here, we report that PEMFs promote bone formation by activating soluble adenylyl cyclase (sAC), cyclic adenosine monophosphate (cAMP), protein kinase A (PKA), and cAMP response element-binding protein (CREB) signaling pathways. First, it was found that 50 Hz 0.6 millitesla (mT) PEMFs promoted osteogenic differentiation of rat calvarial osteoblasts (ROBs), and that PEMFs activated cAMP-PKA-CREB signaling by increasing intracellular cAMP levels, facilitating phosphorylation of PKA and CREB, and inducing nuclear translocation of phosphorylated (p)-CREB. Blocking the signaling by adenylate cyclase (AC) and PKA inhibitors both abolished the osteogenic effect of PEMFs. Second, expression of sAC isoform was found to be increased significantly by PEMF treatment. Blocking sAC using sAC-specific inhibitor KH7 dramatically inhibited the osteogenic differentiation of ROBs. Finally, the peak bone mass of growing rats was significantly increased after 2 months of PEMF treatment with 90 min/day. The serum cAMP content, p-PKA, and p-CREB as well as the sAC protein expression levels were all increased significantly in femurs of treated rats. The current study indicated that PEMFs promote bone formation in vitro and in vivo by activating sAC-cAMP-PKA-CREB signaling pathway of osteoblasts directly or indirectly.
WOS研究方向	Cell Biology ; Physiology
语种	英语
出版者	WILEY
WOS记录号	WOS:000451618500074
内容类型	期刊论文
源URL	[http://119.78.100.223/handle/2XXMBERH/32079]
专题	生命科学与工程学院
作者单位	1.Northwest A&F Univ, Dept Biol, Coll Life Sci, Yanglin, Peoples R China; 2.Lanzhou Univ Technol, Sch Life Sci & Engn, Dept Bioengn, Lanzhou, Gansu, Peoples R China; 3.CPLA, Lanzhou Gen Hosp, Inst Orthopaed, Lanzhou 730050, Gansu, Peoples R China; 4.Univ South Australia, Sansom Inst Hlth Res, Sch Pharm & Med Sci, Adelaide, SA, Australia
推荐引用方式 GB/T 7714	Wang, Yuan-Yuan,Pu, Xiu-Ying,Shi, Wen-Gui,et al. Pulsed electromagnetic fields promote bone formation by activating the sAC-cAMP-PKA-CREB signaling pathway[J]. JOURNAL OF CELLULAR PHYSIOLOGY,2019,234(3):2807-2821.
APA	Wang, Yuan-Yuan.,Pu, Xiu-Ying.,Shi, Wen-Gui.,Fang, Qing-Qing.,Chen, Xin-Ru.,...&Chen, Ke-Ming.(2019).Pulsed electromagnetic fields promote bone formation by activating the sAC-cAMP-PKA-CREB signaling pathway.JOURNAL OF CELLULAR PHYSIOLOGY,234(3),2807-2821.
MLA	Wang, Yuan-Yuan,et al."Pulsed electromagnetic fields promote bone formation by activating the sAC-cAMP-PKA-CREB signaling pathway".JOURNAL OF CELLULAR PHYSIOLOGY 234.3(2019):2807-2821.