Research on the Callus Formation Conditions of the Codonopsis pilosula Induced by Nano-TiO2

doi:10.1166/jbmb.2019.1834

	Research on the Callus Formation Conditions of the Codonopsis pilosula Induced by Nano-TiO2
	Zhao, Ying 1; Zhao, Ping 2; Luo, Junjie 1; Tian, Li 3; Tian, Zhenji 4
刊名	JOURNAL OF BIOBASED MATERIALS AND BIOENERGY
	2019-04
卷号	13 期号:2 页码:247-251
关键词	Codonopsis pilosula Callus Induction Conditions Nano-TiO2
ISSN号	1556-6560
DOI	10.1166/jbmb.2019.1834
英文摘要	A method for induction of the Codonopsis pilosula callus and its subculture was established. Seeds were sterilized with different methods for inducing aseptic seedling. The different parts of plants and C. pilosula aseptic seedlings were used for the explants, and various concentrations of auxin NAA, cytokinin 6-BA and nano-TiO2 combination on MS medium were used for callus induction, then the calluses with better growth status were selected for secondary culture. The best disinfectant was mercuric chloride. Seeds whose germination rate was up to 65.2% were disinfected with 75% of ethanol for 40 s, then disinfected 20 min with 1.5% of NaClO, and then with 0.1% of HgCl2 solution disinfected for 3 min. Aseptic seedling hypocotyl as explants was the best for callus induction, adding 1 mg/L 6-BA and 1.5 mg/L of NAA and 0.02 mg/L of nano-TiO2 to MS medium, and induced rate was nearly 100%. Nano-TiO2 was effective in preventing bacterial contamination of MS medium. Calluses which were induced by cotyledon serving as explants appeared the most severe browning. Thereafter, the optimal hormone combination of C. pilosula subculture was 2 mg/L 6-BA, 1 mg/L NAA and 0.02 mg/L Nano-TiO2.
资助项目	Gansu Agricultural Biotechnology Research and Application Development Project[GNSW-2016-19]
WOS研究方向	Chemistry ; Energy & Fuels ; Materials Science
语种	英语
出版者	AMER SCIENTIFIC PUBLISHERS
WOS记录号	WOS:000473056800015
状态	已发表
内容类型	期刊论文
源URL	[http://119.78.100.223/handle/2XXMBERH/32007]
专题	生命科学与工程学院
通讯作者	Tian, Zhenji
作者单位	1.Gansu Acad Agr Sci, Inst Biotechnol, Lanzhou 730070, Gansu, Peoples R China 2.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Gansu, Peoples R China 3.Univ Manitoba, Fac Agr & Food Sci, Winnipeg, MB R3T 2N2, Canada 4.Lanzhou Univ Technol, Sch Sci, Lanzhou 730050, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Ying,Zhao, Ping,Luo, Junjie,et al. Research on the Callus Formation Conditions of the Codonopsis pilosula Induced by Nano-TiO2[J]. JOURNAL OF BIOBASED MATERIALS AND BIOENERGY,2019,13(2):247-251.
APA	Zhao, Ying,Zhao, Ping,Luo, Junjie,Tian, Li,&Tian, Zhenji.(2019).Research on the Callus Formation Conditions of the Codonopsis pilosula Induced by Nano-TiO2.JOURNAL OF BIOBASED MATERIALS AND BIOENERGY,13(2),247-251.
MLA	Zhao, Ying,et al."Research on the Callus Formation Conditions of the Codonopsis pilosula Induced by Nano-TiO2".JOURNAL OF BIOBASED MATERIALS AND BIOENERGY 13.2(2019):247-251.