Isolation and characterization of phellodendronoside A, a new isoquinoline alkaloid glycoside with anti-inflammatory activity from Phellodendron chinense Schneid | |
Si, Yanpo4; Li, Xiaofei4; Guo, Tao4; Wei, Wenjun; Zhang, Jubao5; Jia, An; Wang, Ya3,5; Zhao, Aihong5; Chang, Jun2; Feng, Shuying1 | |
刊名 | FITOTERAPIA |
2021-10 | |
卷号 | 154 |
关键词 | Phellodendron chinense Phellodendronoside A Anti-inflammatory MAPK pathway Molecular docking |
ISSN号 | 0367-326X |
DOI | 10.1016/j.fitote.2021.105021 |
英文摘要 | Bark of Phellodendron chinense Schneid. (Rutaceae), called Huang Bai in China, is one of the 50 most used Chinese medicines in clinical practice. In this paper, a new isoquinoline alkaloid glycoside was isolated from P. chinense, and its structure was elucidated using spectroscopic method. The compound was eventually identified as (1S, 3 '' S)-1, 2, 3, 4-tetrahydro-7-hydroxy-1-[(4-hydroxybenzyl) methyl]-2-methyl-8-O-isoquinolinyl-[3-hydroxy-3-methylglutaryl]-beta-D-glucopyranoside and named as Phellodendronoside A (PDA). The results of molecular docking showed that PDA could stably bind to an extracellular signal-regulated kinase (ERK), stress-activated protein kinase (JNK) and p38 mitogen-activated protein kinase (p38MAPK) proteins that are closely related to inflammation. Further, the anti-inflammatory activity of PDA was evaluated using the lipopolysaccharide (LPS) induced RAW264.7 macrophage model. We observed that PDA can effectively reduce the levels of nitric oxide (NO), tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), and decrease the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Moreover, we found that PDA inhibits the activation of ERK, JNK and p38MAPK proteins in the MAPK signaling pathway. Collectively, the present study demonstrates that PDA has excellent anti-inflammatory effect in vitro by inhibiting the overproduction of pro-inflammatory mediators, and its mechanism of action involves suppressing the activation of MAPK pathways, suggesting that PDA may be a potential agent for the treatment of inflammatory illness. |
WOS研究方向 | Pharmacology & Pharmacy |
语种 | 英语 |
出版者 | ELSEVIER |
WOS记录号 | WOS:000703820600010 |
内容类型 | 期刊论文 |
源URL | [http://ir.lut.edu.cn/handle/2XXMBERH/149957] |
专题 | 生命科学与工程学院 |
作者单位 | 1.Henan Univ Chinese Med, Coll Med, Zhengzhou 450046, Peoples R China 2.Fudan Univ, Human Phenome Inst, Shanghai 201203, Peoples R China; 3.Huanghe Sci & Technol Coll, Sch Med, Zhengzhou 450063, Peoples R China; 4.Henan Univ Chinese Med, Dept Pharm, Zhengzhou 450046, Peoples R China; 5.Lanzhou Univ technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China; |
推荐引用方式 GB/T 7714 | Si, Yanpo,Li, Xiaofei,Guo, Tao,et al. Isolation and characterization of phellodendronoside A, a new isoquinoline alkaloid glycoside with anti-inflammatory activity from Phellodendron chinense Schneid[J]. FITOTERAPIA,2021,154. |
APA | Si, Yanpo.,Li, Xiaofei.,Guo, Tao.,Wei, Wenjun.,Zhang, Jubao.,...&Feng, Shuying.(2021).Isolation and characterization of phellodendronoside A, a new isoquinoline alkaloid glycoside with anti-inflammatory activity from Phellodendron chinense Schneid.FITOTERAPIA,154. |
MLA | Si, Yanpo,et al."Isolation and characterization of phellodendronoside A, a new isoquinoline alkaloid glycoside with anti-inflammatory activity from Phellodendron chinense Schneid".FITOTERAPIA 154(2021). |
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