Application of enzyme-assisted extraction of baicalin fromScutellaria baicalensisGeorgi

doi:10.1080/10826068.2020.1808791

	Application of enzyme-assisted extraction of baicalin fromScutellaria baicalensisGeorgi
	Ma, Xiao-Di; Zhang, Xin-Guo; Guo, Si-Jia; Ma, Guo-Yan; Liu, Wen-Jie; Wang, Nan; Feng, Ming; Su, Yu
刊名	PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
	2021-03-01
卷号	51 期号:3 页码:241-251
关键词	Assisted extraction baicalin cellulase endophyte Scutellaria baicalensisGeorgi
ISSN号	1082-6068
DOI	10.1080/10826068.2020.1808791
英文摘要	Endophytes may depend on degrading the plant cell wall with cellulases for their survival. Therefore, cellulase produced by endophytes may be useful in releasing the active ingredient of medicinal plants.Scutellaria baicalensisGeorgi is a traditional Chinese medicinal plant widely used in China and baicalin is one of its main active ingredients. In this study, freshS. baicalensisGeorgi was used to isolate endophytes, Congo red staining was used to screen cellulase-producing strains, and HPLC was used to determine the content of baicalin inS. baicalensisGeorgi. As a result, a highly active strain of endophyte capable of the extraction of high levels of baicalin was obtained. The strain was named HG-5 and identified asBacillussp. Scanning electron microscopy analysis confirmed that the enzyme better promotes the dissolution of plant active ingredients. After optimizing the enzyme production and extraction processes, we found that when compared with the traditional extraction method, the baicalin yield was increased 79.31% after extraction with the HG-5 enzyme. The current study provides a novel approach and method for the use of endophyte cellulase to improve the extraction of compounds from medicinal plants.
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
语种	英语
出版者	TAYLOR & FRANCIS INC
WOS记录号	WOS:000561906700001
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/148651]
专题	生命科学与工程学院
作者单位	Lanzhou Univ Technol, Sch Life Sci & Engn, Key Lab Screening & Proc New Tibetan Med Gansu Pr, 287 Langongping Rd, Lanzhou 730050, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Ma, Xiao-Di,Zhang, Xin-Guo,Guo, Si-Jia,et al. Application of enzyme-assisted extraction of baicalin fromScutellaria baicalensisGeorgi[J]. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,2021,51(3):241-251.
APA	Ma, Xiao-Di.,Zhang, Xin-Guo.,Guo, Si-Jia.,Ma, Guo-Yan.,Liu, Wen-Jie.,...&Su, Yu.(2021).Application of enzyme-assisted extraction of baicalin fromScutellaria baicalensisGeorgi.PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,51(3),241-251.
MLA	Ma, Xiao-Di,et al."Application of enzyme-assisted extraction of baicalin fromScutellaria baicalensisGeorgi".PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY 51.3(2021):241-251.