Novel colorimetric aptasensor based on unmodified gold nanoparticle and ssDNA for rapid and sensitive detection of T-2 toxin

doi:10.1016/j.foodchem.2021.129128

	Novel colorimetric aptasensor based on unmodified gold nanoparticle and ssDNA for rapid and sensitive detection of T-2 toxin
	Zhang, Wenwei 2,3; Wang, Yanling 4; Nan, Mina 1; Li, Yongcai 3; Yun, Jianmin 3; Wang, Yi 3; Bi, Yang 3
刊名	FOOD CHEMISTRY
	2021
卷号	348
关键词	T-2 toxin Gold nanoparticles Aptamers Colorimetric aptasensor Detection
ISSN号	0308-8146
DOI	10.1016/j.foodchem.2021.129128
英文摘要	A novel colorimetric aptasensor based on unmodified gold nanoparticle (AuNPs) and single-strand DNA (ssDNA) aptamer was developed for the rapid and sensitive detection of T-2 toxin. In the absence of T-2, the AuNPs were wrapped by the aptamer to avoid the salt-induced aggregation and the solution remains red. In the presence of T2, the aptamer was bound with T-2 and released from the surface of AuNPs, resulting in the aggregation of AuNPs under proper salt solution and the color change from red to purple-blue. The aptasensor exhibited a high sensitivity and selectivity for the detection of T-2. The range of linearity and detection limit were 0.1 ng/mL5000 ng/mL (0.21435 nM-10717.5 nM) and 57.8 pg/mL (0.124 nM), respectively. The aptasensor developed here was applicable to assay T-2 in wheat and corn samples. These results implied that the colorimetric aptasensor was potentially useful in food detection.
WOS研究方向	Chemistry ; Food Science & Technology ; Nutrition & Dietetics
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000623052900005
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/147398]
专题	生命科学与工程学院
作者单位	1.Gansu Agr Univ, Coll Sci, Lanzhou 730070, Peoples R China 2.Gansu Agr Univ, Coll Hort, Lanzhou 730070, Peoples R China; 3.Gansu Agr Univ, Coll Food Sci & Engn, Lanzhou 730070, Peoples R China; 4.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China;
推荐引用方式 GB/T 7714	Zhang, Wenwei,Wang, Yanling,Nan, Mina,et al. Novel colorimetric aptasensor based on unmodified gold nanoparticle and ssDNA for rapid and sensitive detection of T-2 toxin[J]. FOOD CHEMISTRY,2021,348.
APA	Zhang, Wenwei.,Wang, Yanling.,Nan, Mina.,Li, Yongcai.,Yun, Jianmin.,...&Bi, Yang.(2021).Novel colorimetric aptasensor based on unmodified gold nanoparticle and ssDNA for rapid and sensitive detection of T-2 toxin.FOOD CHEMISTRY,348.
MLA	Zhang, Wenwei,et al."Novel colorimetric aptasensor based on unmodified gold nanoparticle and ssDNA for rapid and sensitive detection of T-2 toxin".FOOD CHEMISTRY 348(2021).