Study on Interaction of Coomassie Brilliant Blue G-250 with Bovine Serum Albumin by Multispectroscopic

doi:10.1007/s10989-020-10096-6

	Study on Interaction of Coomassie Brilliant Blue G-250 with Bovine Serum Albumin by Multispectroscopic
	Yang, Mingjun 3; Shi, Dongxiang 3; Wang, Yonggang 3; Ebadi, Abdol Ghaffar 2; Toughani, Mohsen 1
刊名	INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
	2021-03
卷号	27 期号:1 页码:421-431
关键词	SMALL-MOLECULE PROTEIN BINDING METHODOLOGIES FLUORESCENCE SPECTROSCOPY SENSITIVITY EXCITATION MECHANISM ASSAY
ISSN号	1573-3149
DOI	10.1007/s10989-020-10096-6
英文摘要	Aim of present study was to investigate the interaction of coomassie brilliant blue G-250 (CBBG-250) with bovine serum albumin (BSA) by the multispectroscopic methods. Fluorescence-data showed that the complex of BSA-CBBG-250 forming made the intrinsic fluorescence quenching of BSA by CBBG-250 interaction. BSA also could interact with CBBG-250 and the CBBG-BSA complexes formed in a molar ratio of 1:1. UV-Vis results displayed that the apparent binding (association) constantK(a)of CBBG-250 with BSA was 53 x 10(4)(298 K), 34 x 10(4)(303 K), 2.84 x 10(4)(308 K) and 1.99 x 10(4)(313 K) L mol(-1)at different temperatures, respectively. The enthalpy change (oH) and entropy change (oS) were respectively calculated to be - 45.32 kJ mol(-1)and - 139.18 J mol(-1)K(-1), indicating that the hydrogen bonds andVan der Waalsforces played dominant roles in the interaction. The results showed that the diphenylamine structure and amino acid residues in the Coomassie Brilliant Blue G-250 had a strong Van der Waals force. The phenyl sulphonic acid group undergoes electrostatic interactions and hydrogen bond interactions with basic amino acids; the compound Coomassie Brilliant Blue G-250 can form a stable complex with BSA.
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000550659300003
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/147387]
专题	生命科学与工程学院
通讯作者	Wang, Yonggang
作者单位	1.Islamic Azad Univ, Babol Branch, Dept Fishery, Babol Sar, Iran 2.Islamic Azad Univ, Jouybar Branch, Dept Agr, Jouybar, Iran; 3.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China;
推荐引用方式 GB/T 7714	Yang, Mingjun,Shi, Dongxiang,Wang, Yonggang,et al. Study on Interaction of Coomassie Brilliant Blue G-250 with Bovine Serum Albumin by Multispectroscopic[J]. INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS,2021,27(1):421-431.
APA	Yang, Mingjun,Shi, Dongxiang,Wang, Yonggang,Ebadi, Abdol Ghaffar,&Toughani, Mohsen.(2021).Study on Interaction of Coomassie Brilliant Blue G-250 with Bovine Serum Albumin by Multispectroscopic.INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS,27(1),421-431.
MLA	Yang, Mingjun,et al."Study on Interaction of Coomassie Brilliant Blue G-250 with Bovine Serum Albumin by Multispectroscopic".INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS 27.1(2021):421-431.