Effects of patulin on the proliferation and apoptosis of human embryonic kidney cells | |
Zhang, Bai-Gang1,2; Peng, Xiao-Li1; Li, Guang-Hui1; Xu, Yun-Feng1; Xia, Xiao-Dong1 | |
刊名 | Modern Food Science and Technology |
2015-03-15 | |
卷号 | 31期号:3页码:12-17 |
关键词 | Cell death Cell membranes Cell proliferation Cytology Fluorescence Fluorescence microscopy Gene expression Genes Magnesium printing plates Mitochondria Polymerase chain reaction Scanning electron microscopy Toxic materials Dose-dependent manner Human embryonic kidney cells Mitochondrial membrane potential Mitochondrial pathways Morphological changes Patulin Proliferation and apoptosis Real-time fluorescent |
ISSN号 | 16739078 |
DOI | 10.13982/j.mfst.1673-9078.2015.3.003 |
英文摘要 | To study the mechanism of patulin on the proliferation and apoptosis of human embryonic kidney cells, HEK293 cells were cultured and treated with different concentrations of patulin in vitro. The effect of patulin on cell proliferation level was examined by LDH cytotoxicity assay. The morphological changes in patulin-treated HEK293 cells were observed using scanning electron microscopy and fluorescence microscopy. The cell apoptosis rate was detected by Annexin V-EGFP/PI double staining. The mitochondrial membrane potential was measured by a JC-1 probe. The expression levels of the mitochondrial related genes FIS1, ASL, SLC25A6, and COX17 were measured by real-time fluorescent quantitative PCR. The results showed that PAT inhibited the growth of HEK293 cells in a dose-dependent manner and resulted in apoptosis in HEK293 cells. Treatments with 2.5, 5, 7.5, 10, and 15 μM PAT for 8 h resulted in significant decreases in cell viability by 8.1%, 18.2%, 31.0%, 42.2%, and 63.1%, respectively. When treated with 5 μM patulin for 0, 3, 10, and 24 h, the cell survival rates were 94.2%, 78.1%, and 47.4%, respectively. After treatment with 5 and 10 μM PAT for 8 h, the number of apoptotic cells increased in a dose-dependent manner by 16.4% and 20.1%. The changes in the expression levels of mitochondrial membrane potential and mitochondrial genes indicate that patulin may cause HEK293 apoptosis through mitochondrial pathways. ©, 2015, South China University of Technology. All right reserved. |
语种 | 中文 |
出版者 | South China University of Technology |
内容类型 | 期刊论文 |
源URL | [http://ir.lut.edu.cn/handle/2XXMBERH/113106] |
专题 | 生命科学与工程学院 |
作者单位 | 1.College of Food Science and Engineering, Northwest A&F University, Yangling, China; 2.College of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, China |
推荐引用方式 GB/T 7714 | Zhang, Bai-Gang,Peng, Xiao-Li,Li, Guang-Hui,et al. Effects of patulin on the proliferation and apoptosis of human embryonic kidney cells[J]. Modern Food Science and Technology,2015,31(3):12-17. |
APA | Zhang, Bai-Gang,Peng, Xiao-Li,Li, Guang-Hui,Xu, Yun-Feng,&Xia, Xiao-Dong.(2015).Effects of patulin on the proliferation and apoptosis of human embryonic kidney cells.Modern Food Science and Technology,31(3),12-17. |
MLA | Zhang, Bai-Gang,et al."Effects of patulin on the proliferation and apoptosis of human embryonic kidney cells".Modern Food Science and Technology 31.3(2015):12-17. |
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