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	"Modified flocculation" of protein with a combination of copolymer and polyacrylamide
	Yu, J; Liu, HZ; Chen, JY
刊名	CHEMICAL ENGINEERING SCIENCE
	1999-12-01
卷号	54期号:24页码:5839-5843
关键词	flocculation protein anionic copolymer polyacrylamide
ISSN号	0009-2509
其他题名	Chem. Eng. Sci.
中文摘要	Precipitation of soluble proteins in fermentation broth with addition of water-soluble polymers is an important method for downstream processing in biochemical technology. In the present work, the chemical features of "modified flocculation" are explored with lysozyme as the model protein to be precipitated with polyacrylamide in combination of an anionic copolymer containing reactive functional groups. The protein first interacts with low molecular weight anionic copolymer at gentle agitation, followed by precipitation with addition of high molecular weight polyacrylamide. The data on protein recovery in terms of light absorbance (280 nm) are analyzed to shed light on the mechanism of flee formation. The size distribution of the flocs was determined and the effects of the structure of the copolymer on the mechanism of flocculation were investigated. The results support the description of "modified flocculation", which enriches the interaction between protein and polymer, and leads to formation of protein-polymer flocs large enough to precipitate easily in water. (C) 1999 Elsevier Science Ltd. All rights reserved.
英文摘要	Precipitation of soluble proteins in fermentation broth with addition of water-soluble polymers is an important method for downstream processing in biochemical technology. In the present work, the chemical features of "modified flocculation" are explored with lysozyme as the model protein to be precipitated with polyacrylamide in combination of an anionic copolymer containing reactive functional groups. The protein first interacts with low molecular weight anionic copolymer at gentle agitation, followed by precipitation with addition of high molecular weight polyacrylamide. The data on protein recovery in terms of light absorbance (280 nm) are analyzed to shed light on the mechanism of flee formation. The size distribution of the flocs was determined and the effects of the structure of the copolymer on the mechanism of flocculation were investigated. The results support the description of "modified flocculation", which enriches the interaction between protein and polymer, and leads to formation of protein-polymer flocs large enough to precipitate easily in water. (C) 1999 Elsevier Science Ltd. All rights reserved.
WOS标题词	Science & Technology ; Technology
类目[WOS]	Engineering, Chemical
研究领域[WOS]	Engineering
关键词[WOS]	PRECIPITATION ; MECHANISM
收录类别	SCI
原文出处	://WOS:000082678100004
语种	英语
WOS记录号	WOS:000082678100004
公开日期	2013-11-15
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/5996]
专题	过程工程研究所_研究所（批量导入）
作者单位	Chinese Acad Sci, Young Scientist Lab Separat Sci & Engn, Inst Chem Met, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Yu, J,Liu, HZ,Chen, JY. "Modified flocculation" of protein with a combination of copolymer and polyacrylamide[J]. CHEMICAL ENGINEERING SCIENCE,1999,54(24):5839-5843.
APA	Yu, J,Liu, HZ,&Chen, JY.(1999)."Modified flocculation" of protein with a combination of copolymer and polyacrylamide.CHEMICAL ENGINEERING SCIENCE,54(24),5839-5843.
MLA	Yu, J,et al.""Modified flocculation" of protein with a combination of copolymer and polyacrylamide".CHEMICAL ENGINEERING SCIENCE 54.24(1999):5839-5843.

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