Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties | |
Wang, Yonggang4,5; Li, Yuanli4; Li, Shaowei1; Li, QingYang2; Fan, Wenguang5; Kiatoukosin, Lormoua5; Chen, Jixiang3,4 | |
刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES |
2019-07-15 | |
卷号 | 133页码:172-183 |
关键词 | Endophytic fungus exopolysaccharide Production optimization and purification Structure and bioactivities |
ISSN号 | 0141-8130 |
DOI | 10.1016/j.ijbiomac.2019.03.246 |
英文摘要 | The yield, composition, structure and biofunction of microorganism exopolysaccharide (EPS) depended on their genetics and metabolic pathways. An EPS-producing endophytic fungus was isolated from Angelica sinensis planted in Dingxi, Gansu, China, and identified as Alternaria tenuissima Fl. The production optimization, purification and characterization of the purified EPS from A. tenuissima Fl and the in-vitro antioxidant potentials was investigated. Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4. 7H20, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 C, inoculum size of 3%, 180 r/min for 5 d. The EPS purified by ion exchange column chromatography and gel chromatography was a non-reducing sugar and glycoprotein with pyranoid ring by Fourier transform infrared spectroscopy (FT-IR) analysis, displayed a porous network structure by scanning electron microscope (SEM) observation and exhibited a high thermal stability with the degradation temperature of 303.9 C by thermogravimetric analysis (TG/DSC). Molecular weight of the purified EPS was 3.246 x 104 g/mol, and EPS was composed of o-galacturonic acid, rhamnose, o-mannose, glucose, and o-galactose in ratio of 0.45:3.02:3.25:1.0:0.95. The EPS exhibited strong scavenging activity and may be a new source of natural antioxidant. (C) 2019 Elsevier B.V. All rights reserved. |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
语种 | 英语 |
出版者 | ELSEVIER |
WOS记录号 | WOS:000472685700021 |
内容类型 | 期刊论文 |
源URL | [http://ir.lut.edu.cn/handle/2XXMBERH/155334] |
专题 | 生命科学与工程学院 石油化工学院 |
作者单位 | 1.Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Beijing 100101, Peoples R China; 2.Cent South Univ, Xiangya Sch Med, Changsha 410000, Hunan, Peoples R China; 3.Lanzhou Univ Technol, Sch Petrochem Engn, Lanzhou 730050, Gansu, Peoples R China 4.Lanzhou Univ Technol, Sch Energy & Power Engn, Lanzhou 730050, Gansu, Peoples R China; 5.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Gansu, Peoples R China; |
推荐引用方式 GB/T 7714 | Wang, Yonggang,Li, Yuanli,Li, Shaowei,et al. Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2019,133:172-183. |
APA | Wang, Yonggang.,Li, Yuanli.,Li, Shaowei.,Li, QingYang.,Fan, Wenguang.,...&Chen, Jixiang.(2019).Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,133,172-183. |
MLA | Wang, Yonggang,et al."Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 133(2019):172-183. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论