Loading of free radicals on the functional graphene combined with liquid chromatography-tandem mass spectrometry screening method for the detection of radical-scavenging natural antioxidants

doi:10.1016/j.aca.2013.09.041

CORC > 兰州理工大学 > 兰州理工大学 > 石油化工学院

	Loading of free radicals on the functional graphene combined with liquid chromatography-tandem mass spectrometry screening method for the detection of radical-scavenging natural antioxidants
	Wang, Guoying 1,2; Shi, Gaofeng 1,2; Chen, Xuefu 1,2; Chen, Fuwen 2; Yao, Ruixing 2; Wang, Zhenju 2
刊名	ANALYTICA CHIMICA ACTA
	2013-11-13
卷号	802 页码:103-112
关键词	Antioxidants Loading of free radicals Radical-scavenging Functionalized graphene LC-MS Free radical reaction kinetics
ISSN号	0003-2670
DOI	10.1016/j.aca.2013.09.041
英文摘要	A novel free radical reaction combined with liquid chromatography electrospray ionization tandem mass spectrometry (FRR-LC-PDA-ESI/APCI-MS/MS) screening method was developed for the detection and identification of radical-scavenging natural antioxidants. Functionalized graphene was prepared by chemical method for loading free radicals (superoxide radical, peroxyl radical and PAHs free radical). Separation was performed with and without a preliminary exposure of the sample to specific free radicals on the functionalized graphene, which can facilitate reaction kinetics (charge transfers) between free radicals and potential antioxidants. The difference in chromatographic peak areas is used to identify potential antioxidants. The structure of the antioxidants in one sample (Swertia chirayita) is identified using MS/MS and comparison with standards. Thirteen compounds were found to possess potential antioxidant activity, and their free radical-scavenging capacities were investigated. The thirteen compounds were identified as 1,3,5-trihydroxyxanthone 8-O-beta-D glucopyranoside (PD1), norswertianin (PD2), 1,3,5,8-tetrahydroxyxanthone (PD3), 3, 3, 4, 5, 8-penta hydroxyflavone-6-beta-D glucopyranosiduronic acid-&pentopyranose-7-O-glucopyranoside (PD4), 1,5,8-trihydroxy-3-methoxyxanthone (PD5), swertiamarin (PSI), 2-C-beta-D-glucopyranosyl 1,3,7 trihydroxylxanthone (PS2), 1,3,7 trihydroxylxanthone sop]) glucopyranoside (PL1), 1,3,8-trihydroxyl xanthone 5-O-beta-D glucopyranoside (PL2), 1,3,7-trihydroxy-8methoxyxanthone (PL3), 1,2,3-trihydroxy-7,8-dimethoxyxanthone (PL4), 1,8-dihydroxy-2,6-dimethoxy xanthone (PL5) and 1,3,5,8-tetramethoxydecussatin (PL6). The reactivity and SC50 values of those compounds were investigated, respectively. PD4 showed the strongest capability for scavenging PAHs free radical; PL4 showed prominent scavenging capacities in the lipid peroxidation processes; it was found that all components in S. chirayita exhibited weak reactivity in the superoxide radical scavenging capacity. The use of the free radical reaction screening method based on LC-PDA-ESI/APCI-MS/MS would provide a new approach for rapid detection and identification of radical-scavenging natural antioxidants from complex matrices. (C) 2013 Elsevier B.V. All rights reserved.
WOS研究方向	Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000326373300012
状态	已发表
内容类型	期刊论文
源URL	[http://119.78.100.223/handle/2XXMBERH/34591]
专题	石油化工学院
通讯作者	Wang, Guoying
作者单位	1.Lanzhou Univ Technol, Instrumental Anal & Res Ctr, Lanzhou 730050, Peoples R China 2.Lanzhou Univ Technol, Sch Petrochem Engn, Lanzhou 730050, Peoples R China
推荐引用方式 GB/T 7714	Wang, Guoying,Shi, Gaofeng,Chen, Xuefu,et al. Loading of free radicals on the functional graphene combined with liquid chromatography-tandem mass spectrometry screening method for the detection of radical-scavenging natural antioxidants[J]. ANALYTICA CHIMICA ACTA,2013,802:103-112.
APA	Wang, Guoying,Shi, Gaofeng,Chen, Xuefu,Chen, Fuwen,Yao, Ruixing,&Wang, Zhenju.(2013).Loading of free radicals on the functional graphene combined with liquid chromatography-tandem mass spectrometry screening method for the detection of radical-scavenging natural antioxidants.ANALYTICA CHIMICA ACTA,802,103-112.
MLA	Wang, Guoying,et al."Loading of free radicals on the functional graphene combined with liquid chromatography-tandem mass spectrometry screening method for the detection of radical-scavenging natural antioxidants".ANALYTICA CHIMICA ACTA 802(2013):103-112.